Elx808iu ultra microplate reader

Manufactured by Agilent Technologies

Sourced in United States

The ELX808IU Ultra Microplate Reader is a laboratory instrument designed for absorbance-based measurements in microplates. It is capable of performing various types of absorbance-based assays, including endpoint, kinetic, and spectral scanning. The ELX808IU features a high-performance monochromator-based optical system and an advanced detection system to provide accurate and reliable results.

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Lab products found in correlation

405 select plate washer, by Agilent Technologies (1 mentions)

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Microplate reader (6925 citations) ELx808IU (34 citations) ELx808IU microplate reader (10 citations) Ultra Microplate Reader (10 citations)

6 protocols using elx808iu ultra microplate reader

Global DNA Methylation Quantification

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Genome-wide global DNA methylation was assessed in DNA derived from the 185 PBMC samples from our UKAGS resource (Additional file 1: Table S1) using a colorimetric enzyme linked immunosorbent-assay (ELISA) following the manufacturers’ protocol (Epigentek - Methyl flash DNA quantification (colorimetric)). All reactions were performed in duplicate, and for each new 96-well plate, new standardisation controls were conducted. Mixed plate repeats were also performed to assess for potential batch effects. Absorbance (optical density (OD)) was measured with a micro-plate colorimetric spectrophotometer (Bio-Tek ELX808IU Ultra Microplate Reader), and ODs were converted to genome methylation percentage with the use of positive (5 ng of 50% methylated DNA) and negative control (20 ng of un-methylated DNA) reactions, which were also conducted in duplicate.

Toghill B.J., Saratzis A., Freeman P.J., Sylvius N, & Bown M.J. (2018). SMYD2 promoter DNA methylation is associated with abdominal aortic aneurysm (AAA) and SMYD2 expression in vascular smooth muscle cells. Clinical Epigenetics, 10, 29.

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Quantifying Serum Cytokines in DLBCL

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Serum levels of IL-4 and IFN-γ proteins from DLBCL patients at baseline were assessed using corresponding enzyme-linked immunosorbent assay (ELISA) kits (Takara, Dalian, China) according to the manufacturer’s protocols. Briefly, 100 μL of serum samples was added to each well of microwell plates previously coated with a polyclonal anti-IL-4 or IFN-γ antibody, and the plates were incubated for 2 h at room temperature. After a wash with phosphate-buffered saline (PBS), a polyclonal biotin-conjugated IL-4 or IFN-γ antibody was added to each well, and the plates were further incubated for 1 h at room temperature. After washing, streptavidin-horseradish peroxidase (HRP) was added to each well and incubated for 1 h, and then the unbound streptavidin-HRP was washed away. Next, the color reagent for HRP was added to the wells, and the absorbance was measured at 450 nm using an Elx808IU Ultra Microplate Reader (Bio-Tek, Winooski, VT, USA). A standard curve was constructed using the IL-4 or IFN-γ protein standards provided in the kits. Each sample was analyzed in triplicate.

Yin Q., Chen L., Li Q., Mi R., Li Y., Wei X, & Song Y. (2014). Changes of T-lymphocyte subpopulation and differential expression pattern of the T-bet and GATA-3 genes in diffuse large B-cell lymphoma patients after chemotherapy. Cancer Cell International, 14, 85.

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Cytotoxicity Assessment of Powdered Materials on ADMSCs

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The cytotoxicity of suspensions of powdered material on ADMSCs cells was assessed by MTT (3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium bromide) assay. Briefly, ADMSCs at a density of 20,000–40,000 cells/mL were seeded onto 96-well tissue culture plates and cultured in DMEM with 10% FBS. After 24 h of seeding, the medium was replaced by the medium containing device powdered material at different concentrations (0.0005 g/mL, 0.005 g/mL, 0.05 g/mL, and 0.5 g/mL, resp.) and cultured again for 24 h. Cells supplied with culture media alone served as negative control. After the incubation period, the metabolically active cells were quantified using MTT assay and compared with untreated control. Phenol (1%) served as positive control and ADMSCs alone as negative control. For MTT assay, 10 μL of MTT dye (5 mg/mL in PBS) was added to each well in dark and incubated for 3 h at 37°C. The optical density was measured spectrophotometrically following dissolution in dimethyl sulfoxide (DMSO) at 540 nm (Elx 808iu Ultra Microplate Reader, Bio-Tek Instruments, USA).

Gayathri V., Harikrishnan V, & Mohanan P.V. (2016). Integration of Rabbit Adipose Derived Mesenchymal Stem Cells to Hydroxyapatite Burr Hole Button Device for Bone Interface Regeneration. International Journal of Biomaterials, 2016, 1067857.

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Cytotoxicity Assay of DFNPs on Spleen Cells

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The cytotoxicity assay was performed by MTT assay using spleen cells [20 (link)]. The spleen cells were seeded at a density of 20,000 cells/well in a 96-well plate at 37°C in 5% CO₂ atmosphere. After 24 h of culture, 200, 400, 600, 800, and 1000 μg/mL of DFNPs (in triplicate, cells alone as negative control) were added onto the suspension culture of spleen cells. The cells were incubated at 37 ± 1°C for 24 ± 1 h and examined microscopically for morphological changes and quantitated by MTT assay. 20 μL of MTT dye solution (5 mg/mL in phosphate buffer pH 7.4) was added to each well. After 4 h of incubation, the MTT was removed and formazan crystals formed were solubilized with 200 μL of DMSO. The absorbance of each well was read on a microplate reader (ELx 808iu ultramicroplate reader, Bio-Tek instruments, USA) at 540 nm. The relative cell viability (%) was calculated.

Syama S., Gayathri V, & Mohanan P.V. (2015). Assessment of Immunotoxicity of Dextran Coated Ferrite Nanoparticles in Albino Mice. Molecular Biology International, 2015, 518527.

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ELISA Assay for B. miyamotoi

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Immulon 2HB 96-well plates were coated with 100 ng/well of purified B. miyamotoi recombinant BmaA diluted in carbonate bicarbonate coating buffer (90 mM NaHCO₃, 60 mM Na₂CO₃; pH 9.6) and incubated overnight at 4 ° C. The plate wells were subjected to five washes with Tris-buffered saline–Tween 20 [TBS-T; 20 mM Tris, 140 mM NaCl, 2.7 mM KCl, 0.05 % Tween 20 (pH 7.4)] using a BioTek 405 Select plate washer (BioTek, Winooski, VT), followed by addition of 300 μL blocking buffer (3 % fetal bovine serum in TBS-T) for 60 min at room temperature. Post-block, plates were washed, and sera in blocking buffer (1:100) was added to individual wells in duplicate and incubated for 45 min at room temperature. Plates were washed followed by addition of HRP-conjugated goat anti-human IgG Fc, F(ab’)2 fragment (1:5000) (Life Technologies, Carlsbad, CA) and incubated for 45 min. Plates were washed and developed by addition of KPL SureBlue TMB Microwell Peroxidase substrate (100 μL) (Seracare, Milford, MA) for 10 min. Reaction was terminated with 1 N HCL and samples read at 450 nm using an ELx808IU Ultra microplate reader (Biotek, Winooski, VT). Cutoff values to determine positive samples were calculated by 3 standard deviations above the mean optical density for all healthy control samples. Patient samples were assayed in duplicate and replicated 2 times.

MMR vaccination and autism 1998. (1998). BMJ : British Medical Journal, 316(7134), 796.

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Biomarkers of Ovulation: Plasma Hormone Assays

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The plasma progesterone (P 4 , SRB-T-86624), oestradiol (E 2 , SRB-T-87401), follicle stimulating hormone (FSH, MBS-263518), luteinizing hormone (LH, MBS-265390) and inhibin beta A (MBS-015096) concentrations were measured by ELISA (ELX808IU Ultra Microplate Reader; Bio-Tek Instruments Inc., Winooski, VT, USA) system using commercial goat kits. The standard range of the P 4 , E 2 , FSH, LH and inhibin kits used were 0.05-8.0 ng/ml, 1-300 pg/ml, 100-1.56 mlU/ml, 100-1.56 ng/ml and 31.2-1 000 pg/ml, respectively. The inter-assay coefficients of variation (CV s ) for P 4 , E 2 , FSH, LH and inhibin were 2.7, 2.7, ≤ 12, ≤ 12 and ≤ 15%, respectively, and the intra-assay CV s were 4.6, 4.6, ≤ 8, ≤ 8 and ≤ 15%, respectively. The sensitivities of the P 4 , E 2 , FSH, LH and inhibin assays were 0.048 ng/ml, 0.925 pg/ml, 0.5 mlU/ml, 0.5 ng/ml and 5.0 pg/ml, respectively. The plasma P 4 levels (1 ≥ ng/ml) were recorded as an indication of ovulation (Menchaca and Rubianes 2002) .

Doğan İ., Toker M.B., Alçay S., & Küçükşen D.U. (2020). Ovarian follicle dynamics and hormonal changes during early pregnancy in Saanen goats. Veterinární Medicína, 65(1), 8-17.

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