Sodium selenite
Manufactured by Nacalai Tesque
Sourced in Japan
Sodium selenite is a chemical compound with the formula Na2SeO3. It is a white crystalline solid that is soluble in water. Sodium selenite is commonly used in laboratory settings as a source of the element selenium, which is an essential trace mineral for various biological processes.
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Lab products found in correlation
Se methylseleno l cysteine mesecys, by Thermo Fisher Scientific (3 mentions)
Odium selenate, by Fujifilm (2 mentions)
Seleno l methionine, by Nacalai Tesque (2 mentions)
L selenocystine secys2, by Thermo Fisher Scientific (2 mentions)
8 anilino 1 naphthalenesulfonic acid ans, by Merck Group (1 mentions)
5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Sodium selenate, by Fujifilm (1 mentions)
Spectramax abs, by Molecular Devices (1 mentions)
Celltiter 96 aqueous one solution, by Promega (1 mentions)
4 protocols using sodium selenite
1
Comprehensive Selenium Compounds Characterization
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Chemical reagents. Sodium selenite and SeMet were purchased from Nacalai Tesque (Kyoto, Japan). Se-Methylseleno-l -cysteine (MeSeCys) was purchased from Acros Organics (Waltham, MA, USA). l -Selenocystine (SeCys2) and sodium selenate were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) and Wako Pure Chemical Industries, Ltd. (Osaka, Japan), respectively. SeMetO was synthesized based on the method of Kubachka et al. [39 (link)]. 8-Anilino-1-naphthalenesulfonic acid (ANS) and 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) were purchased from Sigma-Aldrich (Shanghai, China). All chemicals were of reagent grade or higher. Ultrapure deionized water (DIW) with 18.3 MΩ cm resistance was used and was prepared using a Milli-Q system (Merck Millipore, Tokyo, Japan).
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2
Bioselenocompounds: Structural Diversity and Biological Significance
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Sodium selenate and potassium selenocyanate (SeCN−) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Sodium selenite and seleno-l -methionine (SeMet) were purchased from Nacalai Tesque (Kyoto, Japan). Se-Methylseleno-l -cysteine (MeSeCys) and l -selenocystine (SeCys2) were purchased from Acros Organics (Waltham, MA) and Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan), respectively. Trimethylselenonium iodide (TMSe+) was purchased from Tri Chemical (Uenohara, Japan). l -Selenohomolanthionine (SeHLan) and 1β-methylseleno-N-acetylgalactosamine (SeSug1) were synthesized in our laboratory in accordance with our previous work [7 ,8 (link)]. The chemical structures of the bioselenocompounds used in this study are shown in Fig. 1 .Fig. 1 ![]()
Structures of bioselenocompounds used in this study. SeCN−: selenocyanate, SeMet: selenomethionine, MeSeCys: Se-methylselenocysteine, SeHLan: selenohomolanthionine, SeCys2: selenocystine, SeSug1: 1β-methylseleno-N-acetylgalactosamine, TMSe+: trimethylselenonium ion.
3
Synthesis of Selenocompounds for Biological Study
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Sodium selenate and potassium selenocyanate (SeCN−) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Sodium selenite and seleno-l -methionine (SeMet) were purchased from Nacalai Tesque (Kyoto, Japan). Se-Methylseleno-l -cysteine (MeSeCys) and l -selenocystine (SeCys2) were purchased from Acros Organics (Waltham, MA, USA) and Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan), respectively. Trimethylselenonium iodide (TMSe+) was purchased from Tri Chemical (Uenohara, Japan). l -Selenohomolanthionine (SeHLan) and Se-methylseleno-N-acetylgalactosamine (SeSug1) were synthesized in our laboratory in accordance with our previous work [6 (link),8 (link)]. The chemical structures of the bioselenocompounds used in this study are shown in Figure 1 .
4
Selenite and Selenate Cytotoxicity Evaluation
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Cells were seeded in 96-well cell culture plates that are coated with poly d -lysine hydrobromide or collagen type I (Iwaki, 4020-010) containing 100 μL of DMEM/5% FBS. After 24 h, the medium was exchanged with serum-free DMEM, and the cells were exposed to sodium selenite (Nacalai Tesque, 31824-02) or sodium selenate (FUJIFILM Wako Pure Chemical Corporation, 191–09242) for 24 h. To each well, 20 μL of CellTiter 96 AQueous One Solution (Promega, G3582) was added, and the cells were incubated at 37 °C in 5% CO2 atmosphere for 1 h. The production of colored formazan from MTS tetrazolium was measured at 490 nm with SpectraMax ABS (Molecular Devices), and cell viability was calculated. Samples were prepared in triplicate or more in each experiment. For the inhibition of Band 3 protein, the selenite exposure was performed in the presence or absence of 25 μM DIDS (4,4’-diisothiocyanatostilbene-2,2’-disulfonic acid, Sigma-Aldrich, D3514). Samples were prepared in quadruplicate in each experiment. Statistical values were calculated from more than eight samples. The Tukey-Kramer test was performed with statsmodels v0.12.1 in Python 3.8.5.
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