Axio imager z1

Cells were seeded on coverslips at a cell density of 10000 cells/well in 24-well plates (300 µL of medium). After overnight incubation at 37 °C and 5% CO₂, cells were treated with AgNWs. At scheduled time points, cells on coverslips were fixed directly with paraformaldehyde (4%) in PBS (pH 7.4) for 15 min, washed with PBS, permeabilized for 10 min in 0.1% Triton X-100/PBS, washed twice with PBS, blocked with 2% bovine serum albumin (BSA) in PBS for 1 h, washed twice with PBS, stained with Phalloidin Dylight 550 in PBS (2 units/mL, stock solution 300 units/mL in methanol) for 1 h (300 µL, at room temperature), washed twice with PBS, stained with DAPI 2 µg/mL in PBS for 10 min and finally rinsed again with PBS. Coverslips were mounted on glass slides using PermaFluor Aqueous Mounting Medium. Samples were kept protected from light until imaging. Photos were taken with a fluorescence microscope (Zeiss Axio Imager Z1) equipped with a Hamamatsu HR camera and a color AxioCam MRc camera. Images were processed by Volocity 6.3 software.