Log In Sign up
The largest database of trusted experimental protocols

Ifn γ 4s b3

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

The IFN-γ (4S.B3) is a laboratory equipment product. It functions as a recombinant protein.

Automatically generated - may contain errors

Lab products found in correlation

Intracellular staining kit, by Thermo Fisher Scientific (1 mentions) Cd25 bc96, by Thermo Fisher Scientific (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) Lsrfortessa, by BD (1 mentions) Ionomycin, by Merck Group (1 mentions) Golgistop, by BD (1 mentions) Foxp3 pch101, by Thermo Fisher Scientific (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Facscalibur, by BD (1 mentions) Hcd56, by BioLegend (1 mentions) Ebiocb16, by Thermo Fisher Scientific (1 mentions) Icrf44, by BioLegend (1 mentions) Hit3a, by BioLegend (1 mentions) Il 17a, by BioLegend (1 mentions) Cd11b, by BioLegend (1 mentions) Pzap70 py319, by Cell Signaling Technology (1 mentions) P p44 42 mapk t202 y204, by Cell Signaling Technology (1 mentions) α tubulin, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Zap70, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

IFN-γ (1815 citations) Anti-IFN-γ (4S.B3, (3 citations) IFN-γ-PE-Cy7 (clone 4S.B3) (2 citations) FITC-conjugated anti-IFN-γ (clone 4S.B3; (2 citations)

8 protocols using ifn γ 4s b3

1

Multiparameter Flow Cytometry of Activated PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs were immunostained using various combinations of the following fluorescence-conjugated antibodies: CD4 (RPA-T4; BioLegend), CD25 (BC96; eBioscience), Foxp3 (PCH101; eBioscience), IL-17 (eBio64DEC17; eBioscience) and IFN-γ (4S.B3; eBioscience). The cells were also intracellularly stained with the following antibodies: IL-17, IFN- γ and Foxp3. Before intracellular staining, cells were stimulated in culture medium containing 25 ng/mL phorbol myristate acetate (Sigma-Aldrich), 250 ng/mL ionomycin (Sigma-Aldrich) and 1 μL/mL GolgiSTOP (BD Pharmingen) in an incubator with 5% CO2 at 37 °C for 4 h. Intracellular staining was conducted using an intracellular staining kit (eBioscience) according to the manufacturer’s protocol. Flow cytometric analysis was performed on a LSRFortessa (BD Pharmingen).
Song Y., Lim J.Y., Lim T., Im K.I., Kim N., Nam Y.S., Jeon Y.W., Shin J.C., Ko H.S., Park I.Y, & Cho S.G. (2020). Human mesenchymal stem cells derived from umbilical cord and bone marrow exert immunomodulatory effects in different mechanisms. World Journal of Stem Cells, 12(9), 1032-1049.
+ Open protocol
+ Expand
2

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following fluorescence-conjugated antibodies were used for multiparameter flow cytometry: CD3 (SK7), CD4 (SK3), CD8 (SK1), CD14 (M5E2), CD19 (HIB19) CD25 (2A3), IL-10 (JES3-19F1), TNF-α (Mab11), IL-2 (5344.111), γδ TCR (B1) (BD Biosciences, San Jose, CA); CD4 (SFCI12T4D11) (Beckman Coulter, Fullerton, CA); IL-17 (eBio64DEC17) and αβ TCR (IP26) (BioLegend, San Diego, CA); IFN-γ (4S.B3) (eBioscience, San Diego, CA). All antibodies were titrated prior to use to determine optimal staining concentrations. Flow cytometry data was acquired either on a FACS Calibur or LSR-II flow cytometer (BD Biosciences) and data analyzed using FlowJo software (TreeStar, Ashland OR).
Lakhal-Naouar I., Slike B.M., Aronson N.E, & Marovich M.A. (2015). The Immunology of a Healing Response in Cutaneous Leishmaniasis Treated with Localized Heat or Systemic Antimonial Therapy. PLoS Neglected Tropical Diseases, 9(10), e0004178.
+ Open protocol
+ Expand
3

Multiparameter FACS Staining for Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
For FACS staining, we used the following antibodies to target: CD16 (FITC; eBioCB16), CD14 (PE-Cy7; 61D3), CD15 (APC; MMA), IL-4 (APC; 8D4-8), and IFN-γ (4S.B3)—all purchased from eBioscience (San Diego, CA, USA)—and CD11b (FITC; ICRF44), CD56 (PE; HCD56), CD33 (PE; WM53), IL-10 (PE; JES3-9D7) CD45 (APC; HI30), CD163 (APC; GH1/61), CD3 (PE-Cy7; HIT3a), CD4 (FITC; OKT4), and IL-17A (PE; BL168)—all purchased from BioLegend (San Diego, CA, USA).
Kasper M., Walscheid K., Laffer B., Bauer D., Busch M., Wildschütz L., Wang B., Loser K., Vogl T., Grajewski R.S., Langmann T, & Heiligenhaus A. (2018). The Phenotype of Monocytes in Anterior Uveitis Depends on the HLA-B27 Status. Frontiers in Immunology, 9, 1773.
+ Open protocol
+ Expand
4

Characterization of T Cell Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against HLA-A2 (BB7.2), HLA-ABC (W6/32), CD8a (RPA-T8), TNF (MAb11), and IFN-γ (4 S.B3) were purchased from eBioscience. Anti-CD3 (SK7), CD107a (H4A3), CD247-Alexa Fluor 488 (pY142) (K25-407.69), pCD3ζ (pY142) (K25-407.69), Erk1 (Cat# 610031), and PLC-γ1 (Cat# 610027) were from Becton Dickinson. Anti-pLAT (Tyr191) (Cat# 3584S), P-p44/42 MAPK (T202/Y204) (Cat# 4370L), pSrc family (pY416) (Cat# 6943T), non-pSrc family (pY416) (Cat# 2102S), pSHP-1 (Cat# 8849S), pZap70 (pY319) (Cat# 2717S), Zap70 (Cat# 2709), pPLC-γ1 (pY783) (Cat# 2821S), α-tubulin (Cat# 3873S), GAPDH (Cat# 2118S) antibodies were from Cell Signaling Technologies. Anti-LAT (11B.12) and β-2-microglobulin (2M2) antibodies were from Biolegend. Mouse anti-human HLA-A2-E183-91 and mouse antihuman HLA-A2-C18-27 primary antibodies were produced as described18 (link). F(ab’)2-goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (Cat# A-11070), and goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 647 (Cat# A-21237) were purchased from Thermo Fisher Scientific.
Zhao X., Sankaran S., Yap J., Too C.T., Ho Z.Z., Dolton G., Legut M., Ren E.C., Sewell A.K., Bertoletti A., MacAry P.A., Brzostek J, & Gascoigne N.R. (2018). Nonstimulatory peptide–MHC enhances human T-cell antigen-specific responses by amplifying proximal TCR signaling. Nature Communications, 9, 2716.
+ Open protocol
+ Expand
5

Detailed Flow Cytometry Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for flow cytometry. For mouse cells, CD4 (RM4-5; eBioscience), CD8a (53-6.7; BD Biosciences), CD90.2 (53-2.1, eBioscience), PD-1 (29F.1A12; BioLegend), CTLA-4 (UC10-4B9, eBioscience), CD25 (PC61; BD Biosciences), CD69 (H1.2F3; BD Biosciences), Foxp3 (FJK-16s; eBioscience), and c-Maf (sym0F1, eBioscience) were used. For human cells, CD4 (OKT4; BioLegend), CXCR4 (12G5; eBioscience), CCR7 (G043H7; BioLegend), CD127 (A019D5; BioLegend), IFN-γ (4S.B3; eBioscience), Foxp3 (236A/E7; eBioscience) CD8 (PRA-T8, BD Biosciences), CTLA4 (BNI3; BioLegend), TIGIT (MBSA43; eBioscience), CD45 (HI30, BD Biosciences), and PD-1 (MIH4; eBioscience) were used.
Seki A, & Rutz S. (2018). Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells. The Journal of Experimental Medicine, 215(3), 985-997.
+ Open protocol
+ Expand
6

Characterizing Melanoma-Reactive CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood mononuclear cell (PBMC) samples were collected from patients with melanoma. Antibodies for CD8, CD11a (HI111), PD-1 (EH12.2H7) were purchased from BioLegend and IFN-γ (4S.B3) was purchased from eBioscience. The tumor antigen specificity of CD11ahiPD-1+CD8+ T cells was confirmed by staining with HLA-A2/MART-1 tetramer. The function of human tumor-reactive CD8+ T cells was measured by intracellular staining for IFN-γ. Briefly, lymphocytes were incubated with MART-1 and gp100 (Mayo Clinic Peptide Core) at 1 μg/ml of each or PMA (50 ng/ml)/ionomycin (500 ng/ml) for 5 hours. After incubation, cells were stained for surface markers followed by intracellular staining for IFN-γ. To enrich tumor antigen-specific CD8+ T cells, PBMCs of melanoma patients were incubated with antigen peptides for 1 week in complete RPMI medium containing human IL-2 (10 U/ml, Proleukin, Mayo Pharmacy) before functional analysis.
Dronca R.S., Liu X., Harrington S.M., Chen L., Cao S., Kottschade L.A., McWilliams R.R., Block M.S., Nevala W.K., Thompson M.A., Mansfield A.S., Park S.S., Markovic S.N, & Dong H. (2016). T cell Bim levels reflect responses to anti–PD-1 cancer therapy. JCI Insight, 1(6), e86014.
+ Open protocol
+ Expand
7

Comprehensive T Cell Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for flow cytometry analysis: CD3 (SK7; BD), CD4 (SK3; eBioscience), CD8 (RPA-T8; BD), CD69 (FN50; BioLegend), TNF (MAb11; eBioscience), IFN-γ (4S.B3; eBioscience), and MHC I (W6/32; Bio X Cell). The following functional grade antibodies were used to stimulate T cells in culture: CD28 (L293; BD), CD49d (L25; BD), and CD3 (SK7; eBioscience). The pan–anti–MHC II–blocking antibody Tu39 and isotype control IgG2a κ were from BioLegend. The anti–HLA-DR–blocking antibody G46-6 was from BD. The 15mer-overlapping peptide pool covering Ad169 IE-1 and custom-made 15mer-overlapping peptide pools covering the Towne and Toledo IE-1 sequences were from JPT Peptide Technologies. Individual peptides used for truncation studies and for pulsing HLA transfectants were synthesized by JPT or Genemed Synthesis Inc. HLA-B08 tetramers folded with the EM9 and QV9 peptides were from the National Institutes of Health tetramer core facility. Recombinant human IL-2, IL-4, and GM-CSF were from Immunex.
Murray S.E., Nesterenko P.A., Vanarsdall A.L., Munks M.W., Smart S.M., Veziroglu E.M., Sagario L.C., Lee R., Claas F.H., Doxiadis I.I., McVoy M.A., Adler S.P, & Hill A.B. (2017). Fibroblast-adapted human CMV vaccines elicit predominantly conventional CD8 T cell responses in humans. The Journal of Experimental Medicine, 214(7), 1889-1899.
+ Open protocol
+ Expand
8

Quantifying Cytokine Release in iNKT Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
To analyze cytokine release through iNKT cell transactivation, 5 × 106 PBMC from SSc patients or healthy donors were incubated in a 24-well plate together with 100 ng/ml α-GalCer (Sigma-Aldrich) in iNKT cell culture medium consisting of RPMI 1640 GlutaMAX™ Medium (ThermoFisher Scientific), 10% FBS (Biochrom), 100 U/ml PenStrep (Lonza), 5.5 μM 2-mercaptoethanol (Roth), 0.1 mM non-essential amino acids (NEAA; Gibco), 10 mM HEPES (Gibco), and 1 mM sodium pyruvate (Gibco).
To assess intracellular cytokines, cells were stimulated with 1x Cell Stimulation Cocktail (eBioscience) for 4 h at 37 °C and 5% CO2 in iNKT cell culture medium. After staining surface antigens, cells were fixed and permeabilized (eBioscience) prior to staining of intracellular interferon gamma (IFN-γ) (4S.B3, eBioscience), interleukin (IL)-4 (8D4-8, BioLegend), and IL-17 (A019D5, BioLegend). Stained cells were measured using a LSR Fortessa flow cytometer (BD Biosciences), and analysis was performed with FlowJo 10.2 (Tree Star).
Pecher A.C., Kettemann F., Asteriti E., Schmid H., Duerr-Stoerzer S., Keppeler H., Henes J.C., Klein R., Hinterleitner C., Secker K.A., Schneidawind C., Kanz L, & Schneidawind D. (2019). Invariant natural killer T cells are functionally impaired in patients with systemic sclerosis. Arthritis Research & Therapy, 21, 212.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!