The catecholamine epinephrine hydrochloride (EPI), the NOS inhibitors S-methylisothiourea hemisulfate salt (SMIS) and aminoguanidine hemisulfate salt (AGH) as well as the NO donor sodium nitroprusside dihydrate (SNP) were purchased from Sigma-Aldrich. The irreversible inhibitor of soluble guanylyl cyclase 1H-[1,2,4]Oxadiazole[4,3-a]quinoxalin-1-one (ODQ) and the NO donor 3-morpholinosydnonimine chloride (SIN-1) were obtained from AdipoGen Life Sciences and the NOS inhibitors L-NG-nitroarginine methyl ester (L-NAME) hydrochloride, L-NG-nitroarginine (L-NNA) and 7-nitroindazole (7-NI) were purchased from Cayman chemicals. Additional chemicals were Levodopa (L-DOPA), obtained from Santa Cruz Biotechnology, as well as (+)-MK 801 maleate (MK-801) and Ifenprodil (+)-tartrate salt (ifenprodil), both obtained from Selleckchem. Stock solutions at 10− 1 M for SMIS, AGH and SNP or at 10− 2 M for remaining compounds were either prepared with autoclaved Milli-Q dH2O or with DMSO (Sigma-Aldrich) for 7-NI and ODQ. Working solutions (10x concentrate of final concentration of treatment) for each compound were prepared prior to experiments with 1 μm filtered fresh seawater (FSW).
L ng nitroarginine l nna
Manufactured by Cayman Chemical
Sourced in France
L-NG-nitroarginine (L-NNA) is a nitric oxide synthase inhibitor. It is a synthetic compound used in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Epinephrine hydrochloride, by Merck Group (1 mentions)
Sodium nitroprusside dihydrate, by Merck Group (1 mentions)
Aminoguanidine hemisulfate salt, by Merck Group (1 mentions)
S methylisothiourea hemisulfate salt, by Merck Group (1 mentions)
Metergoline, by Bio-Techne (1 mentions)
Yohimbine, by Bio-Techne (1 mentions)
Diazepam, by Roche (1 mentions)
2 protocols using l ng nitroarginine l nna
1
Marine organism chemical treatment protocol
2
Acute Toxicity Evaluation of Adansonia digitata
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The followings are some of the chemicals used for the experiment.
Imipramine (Tofranil GSK brand), diazepam (Roche, France), methanol (Fluka-Aldrich), metergoline (Tocris Bioscience), prazosin (Taiwan Healthcare), yohimbine (Tocris Bioscience), sulpiride (Shengheng Renyoung Pharmaceuticals), cyproheptadine (Advacare Pharma), L-arginine (Puritan's Pride), L-NG-Nitroarginine (L-NNA) (Cayman Chemical) Acute toxicity study Median lethal dose (LD 50 ) was determined using Organization for Economic Co-operation and Development (OECD 425) guidelines in mice. Briefly, two groups each consisting of three mice were fasted 3 hours prior to dosing. Food was further withheld for 1-2 hours after administration of methanol stem bark extract of Adansonia digitata (MEAD). Limit test was conducted in two stages. In the first stage, each mouse was treated with the extract at a dose of 5000 mg/kg and observed for 48 hours. On survival, the second stage was carried out with two additional mice. Mice were observed each during the first 30 minutes of treatment and occasionally within 24 hours finally daily for 14 days.
Imipramine (Tofranil GSK brand), diazepam (Roche, France), methanol (Fluka-Aldrich), metergoline (Tocris Bioscience), prazosin (Taiwan Healthcare), yohimbine (Tocris Bioscience), sulpiride (Shengheng Renyoung Pharmaceuticals), cyproheptadine (Advacare Pharma), L-arginine (Puritan's Pride), L-NG-Nitroarginine (L-NNA) (Cayman Chemical) Acute toxicity study Median lethal dose (LD 50 ) was determined using Organization for Economic Co-operation and Development (OECD 425) guidelines in mice. Briefly, two groups each consisting of three mice were fasted 3 hours prior to dosing. Food was further withheld for 1-2 hours after administration of methanol stem bark extract of Adansonia digitata (MEAD). Limit test was conducted in two stages. In the first stage, each mouse was treated with the extract at a dose of 5000 mg/kg and observed for 48 hours. On survival, the second stage was carried out with two additional mice. Mice were observed each during the first 30 minutes of treatment and occasionally within 24 hours finally daily for 14 days.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!