Iplabs software

Manufactured by BD

Sourced in United States

IPLABS software is a data analysis and visualization tool designed for laboratory environments. It provides functionalities for managing and analyzing experimental data. The software's core function is to facilitate the processing, presentation, and interpretation of laboratory data.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Oct3 4 antibody, by Santa Cruz Biotechnology (1 mentions) Nanog antibody, by Abcam (1 mentions) Gelatin coated chamber slides, by Thermo Fisher Scientific (1 mentions) Anti mouse igg alexa 488 conjugate antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Alexa fluor 555 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Superfrost plus, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

IPLab software IPlab 3.7v software IPLab imaging software IPLab

3 protocols using iplabs software

Immunofluorescence Microscopy of Survivin and Tubulin

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Cells grown on glass coverslips were treated with either DMSO or PTX for 10 h and fixed with 3.7% formaldehyde. The cells were permeabilized with phosphate buffered saline (PBS) containing 0.1% Triton-X100, and blocked with 10% bovine serum albumin diluted in PBS. The cells were incubated with a Survivin (Catalog No. NB500-201; Novus Biologicals, Littleton, CO, USA) or tubulin antibody (Cell Signaling, Catalog No. 2148). The primary antibodies were detected using an Alexa Fluor 488-conjugated secondary antibody (Catalog No. A-11034; Molecular Probes, Eugene, OR, USA), and the cells were also stained with DAPI (Sigma) to label nuclei. Digital images of the cells were captured using a Zeiss fluorescence microscope outfitted with a Sensicam qe camera (Cooke Co., Campbell, CA, USA) and processed using IPLABS software (BD Biosciences, San Jose, CA, USA).

Kreger B.T., Johansen E.R., Cerione R.A, & Antonyak M.A. (2016). The Enrichment of Survivin in Exosomes from Breast Cancer Cells Treated with Paclitaxel Promotes Cell Survival and Chemoresistance. Cancers, 8(12), 111.

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Immunofluorescent Analysis of Pluripotency Markers

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Cells and blastocysts were plated on 0.1% gelatin-coated chamber slides (Thermo Fisher Scientific), treated as indicated, and then fixed with 3.7% formaldehyde diluted in PBS for 30 minutes. The slides were permeabilized with 0.1% Triton X-100 diluted in PBS, blocked with 10% BSA diluted in PBS, and incubated with an Oct3/4 antibody (Santa Cruz Biotechnology) and a Nanog antibody (Abcam) diluted in 5% BSA in PBS at a 1:200 dilution, for 2 hours. The slides were washed with PBS, and then were incubated with an anti-mouse IgG-Alexa 488 conjugate antibody (Thermo Fisher Scientific) and an anti-rabbit IgG-Alexa 548 conjugate antibody (Thermo Fisher Scientific) diluted in 5% BSA in PBS at a 1:400 dilution, for 1 hour. The slides were again washed with PBS and visualized by brightfield and fluorescent microscopy. All images were captured using IPLABS software (BD Biosciences) and processed using ImageJ software.

Hur Y.H., Feng S., Wilson K.F., Cerione R.A, & Antonyak M.A. (2020). Embryonic Stem Cell-Derived Extracellular Vesicles Maintain ESC Stemness by Activating FAK. Developmental cell, 56(3), 277-291.e6.

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Immunofluorescence Staining of Lung and Airway Tissue

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Small specimens of peripheral lung (z0.4 cm 3 ) or trachea (z0.25 cm 2 ) were dissected, embedded in freezing media, immediately frozen in isopentane at 280 8 C, and stored at 280 8 C. Frozen specimens were sectioned (z5-7 mm) at 220 8 C and thaw-mounted on glass slides (Fisher Superfrost/Plus, Pittsburgh, PA). Before labeling, sections were dehydrated in 220 8 C acetone for 5 minutes, treated with 1.5% sodium borohydrate in PBS for 20 minutes to reduce autofluorescence, and then blocked with 3% BSA in PBS for 30 minutes. Sections were incubated with primary antibodies against NKCC1 (NT or T84 [24] ), Mucin 5AC (MUC5AC) (Invitrogen, Carlsbad, CA), or anti-zonula occludens-1 (ZO-1) (Zymed; Invitrogen) for 2 hours at 4 8 C, followed by the appropriate secondary antibodies (Alexa Fluor 488 goat anti-rabbit and Alexa Fluor 555 goat anti-mouse; Life Technologies, Eugene, OR) for 1 hour. After four rinses with PBS, nuclei were stained with 49,6-diamidino-2-phenylindole (Roche, Indianapolis, IN) and specimens were mounted with antifade reagent. Confocal images were digitized with IP Labs software (BD Bioscience, San Jose, CA). Images were visualized individually or assembled as a collage. Antibody specificity was verified by processing paired sections without primary antibody (Figure E4).

Flores-Delgado G., Lytle C, & Quinton P.M. (2016). Site of Fluid Secretion in Small Airways. American journal of respiratory cell and molecular biology, 54(3).

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