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Ncs 3500rs ultimate 3000

Manufactured by Thermo Fisher Scientific
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The NCS-3500RS UltiMate 3000 is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It features a modular design, allowing for customization to meet specific research or laboratory needs. The system includes a pump, autosampler, and detector modules for efficient and reliable separation and detection of a wide range of analytes.

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Lab products found in correlation

Velos pro orbitrap elite, by Thermo Fisher Scientific (3 mentions) Easy source, by Thermo Fisher Scientific (3 mentions) Easy spray column, by Thermo Fisher Scientific (3 mentions) Pepmap rslc c18 material, by Thermo Fisher Scientific (3 mentions) Tgx criterion gradient sds polyacrylamide gels, by Bio-Rad (1 mentions)

Close spelling variants of this product

NCS-3500RS Ultimate 3000 Binary Rapid system Ultimate 3000

3 protocols using ncs 3500rs ultimate 3000

1

Muscle Proteome Analysis by LC-MS/MS

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Muscle lysate protein (80 µg total protein) was separated on 4–20% TGX Criterion gradient SDS polyacrylamide gels (Bio-Rad, Berkeley, CA) and processed for mass spectrometry, as previously described (12 (link)). Mass spectrometry (liquid chromatography–electrospray ionization–tandem mass spectrometry [LC-ESI-MS/MS]) was performed on a Thermo Electron (San Jose, CA) Orbitrap Elite Velos Pro fitted with an EASY source (Thermo Electron, San Jose, CA). NanoLC was performed using a DIONEX/Thermo NCS-3500RS UltiMate 3000 with an EASY Spray column (Thermo Electron, San Jose, CA; 50 cm × 75 μm inner diameter, packed with PepMap RSLC C18 material, 2 μm). A “top 15” data-dependent MS/MS analysis was performed (acquisition of a full-scan spectrum followed by collision-induced dissociation mass spectra of the 15 most abundant ions in the survey scan).
Campbell L.E., Langlais P.R., Day S.E., Coletta R.L., Benjamin T.R., De Filippis E.A., Madura JA I.I., Mandarino L.J., Roust L.R, & Coletta D.K. (2016). Identification of Novel Changes in Human Skeletal Muscle Proteome After Roux-en-Y Gastric Bypass Surgery. Diabetes, 65(9), 2724-2731.
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2

HPLC-ESI-MS/MS for Protein Identification

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HPLC-ESI-MS/MSn was performed on a Thermo Electron Orbitrap Elite Velos Pro fitted with an EASY source (Thermo Electron, San Jose, CA). NanoLC was performed using a DIONEX/Thermo NCS-3500RS UltiMate 3000 with an EASY Spray column (Thermo Electron, 50cm × 75-um inner diameter, packed with PepMap RSLC C18 material, 2 um); loading phase for 15 min; mobile phase, linear gradient of 1–37% ACN in 0.1% FA in 150 min, followed by a step to 95% ACN in 0.1% FA over 5 min, hold 10 min, and then a step to 1% ACN in 0.1% FA over 1 min and a final hold for 19 min (total run 200 min); Buffer A = 0.1% FA in 100% H2O; Buffer B = 0.1% FA in 100% ACN; flow rate, 300 nl/min. All solvents were mass spectrometry grade. A “top 15” data-dependent MS/MS analysis was performed (acquisition of a full scan spectrum followed by collision-induced dissociation mass spectra of the 15 most abundant ions in the survey scan).
Kras K.A., Willis W.T., Barker N., Czyzyk T., Langlais P.R, & Katsanos C.S. (2016). Subsarcolemmal mitochondria isolated with the proteolytic enzyme nagarse exhibit greater protein specific activities and functional coupling. Biochemistry and Biophysics Reports, 6, 101-107.
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3

Proteomic Analysis by HPLC-ESI-MS/MS

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HPLC-ESI-MS/MSn was performed on a Thermo Electron Orbitrap Elite Velos Pro fitted with an EASY source (Thermo Electron, San Jose, CA). NanoLC was performed using a DIONEX/Thermo NCS-3500RS UltiMate 3000 with an EASY Spray column (Thermo Electron, 50 cm×75-µm inner diameter, packed with PepMap RSLC C18 material, 2 μm); loading phase for 15 min; mobile phase, linear gradient of 1–37% ACN in 0.1% FA in 150 min, followed by a step to 95% ACN in 0.1% FA over 5 min, hold 10 min, and then a step to 1% ACN in 0.1% FA over 1 min and a final hold for 19 min (total run 200 min); Buffer A=0.1% FA in 100% H2O; Buffer B=0.1% FA in 100% ACN; flow rate, 300 nl/min. All solvents were mass spectrometry grade. A “top 15″ data-dependent MS/MS analysis was performed (acquisition of a full scan spectrum followed by collision-induced dissociation mass spectra of the 15 most abundant ions in the survey scan).
Kras K.A., Willis W.T., Barker N., Czyzyk T., Langlais P.R, & Katsanos C.S. (2016). Subsarcolemmal mitochondria isolated with the proteolytic enzyme nagarse exhibit greater protein specific activities and functional coupling. Biochemistry and Biophysics Reports, 6, 101-107.
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