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Goat anti mouse igg fitc antibody

Manufactured by Thermo Fisher Scientific

Goat anti-mouse IgG-FITC antibody is a secondary antibody conjugated with the fluorescent dye fluorescein isothiocyanate (FITC). It is used to detect and visualize primary antibodies that are raised in mouse. The FITC label allows for fluorescent detection and imaging of target proteins or cells.

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2 protocols using goat anti mouse igg fitc antibody

1

Flow Cytometric Analysis of PAR1 Expression

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Cells were detached using trypsin and pelleted, resuspended and washed
with 100 μL PBS 3 times. Cells were then incubated at 4 °C for
30 minutes with PAR1 mouse monoclonal antibody (Invitrogen), and washed 3 times
with PBS. Cells were then incubated with goat anti-mouse IgG-FITC antibody
(Invitrogen) for 30 minutes at 4 °C, and washed 3 times with PBS. Cells
were then fixed in 2% formaldehyde and cells were analyzed using a
BDFacs Canto II flow cytometer (BD Biosciences, San Jose, CA) equipped with a
488 nm argon laser and a 530 bandpass filter (FL1). A minimum of 10,000 events
were counted for each data point. The data was analyzed using the FACSDiva
version 6.1.1 software. Fluorescence data is expressed as mean arbitrary
fluorescence units and were gated to include all healthy cells.
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2

Immunofluorescence Detection of FOXP3

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The expression of FOXP3 in Hep3B and 97H cells was detected by immunofluorescence, which was performed as previously described [22 (link)], using mouse anti-human FOXP3 Ab (1:200 dilution, Santa Cruz Biotechnology) and a goat anti-mouse IgG-FITC antibody (Invitrogen). Images were acquired using a LSM510 Confocal Laser Scanning Microscope (Carl Zeiss).
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