Hydrochloric acid (HCl) and l -(+)-Ascorbic acid were purchased from Merck (Darmstadt, Germany). Na2CO3 and Na2SO3 were purchased from Riedel de Haen (Seelze, Germany). Chloroform (CHCl3) was purchased from LAB-SCAN (Dublin, Ireland). Oleic acid and Iron(II) sulfate heptahydrate (FeSO4·7H2O) were purchased from Sigma Aldrich (Steinheim, Germany). Iron(III) chloride anhydrous was purchased from Fluka Chemicals (Buchs, Switzerland). NaOH was from Pronalab (Madalena, Portugal). Ethanol was from Panreac Quimica (Barcelona, Spain). The medium brain-heart infusion (BHI) and Bacteriological agar type E were purchased from Biokar Diagnostics (Beauvais, France).
Bacteriological agar type e
Manufactured by Solabia
Sourced in France
Bacteriological agar type E is a solidifying agent used in microbiological culture media. It is a purified form of agar, a polysaccharide extracted from certain red algae. Bacteriological agar type E provides a stable, transparent gel that supports the growth of a wide range of microorganisms.
Automatically generated - may contain errors
2 protocols using bacteriological agar type e
1
Synthesis and Characterization of Iron Nanoparticles
2
Stagonosporopsis cucurbitacearum Fungal Cultivation
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Stagonosporopsis cucurbitacearum isolate 04.14/006 was isolated from infected cucumbers on a Belgian cucumber plantation by the Sint-Katelijne-Waver Research Station for Vegetable Production, Belgium (PSKW) and identified by Scientia Terrae, Sint-Katelijne-Waver, Belgium. To obtain a stock, the isolate was stored at -80 °C in the fungal collection of the Laboratory of Sustainable Plant Production and Protection, KU Leuven, Geel. In preparation to perform experiments, the isolate was maintained and propagated on Potato Dextrose Agar (PDA, Biokar Diagnostics, Beauvais Cedex, France) at 23 °C in a 12 h light (PPFD: 10 µmol m -2 s -1 ) / 12 h dark regime for 14 days.
PDA is the most widely used medium for growing fungi and bacteria. Due to its semisynthetic composition, variations are possible in each batch. Therefore, a synthetic medium was also selected to conduct the experiments. To this minimal medium (MM), based on the Czapek-Dox recipe by Leslie and Summerel (2006) , 20 g of sucrose and 2 g NaNO3 was added per litre medium. For the experiments conducted on solid media, 15 g agar (Bacteriological Agar Type E, Biokar Diagnostics, Beauvais Cedex, France) per litre was added to the different minimal media. PDA was used as the reference medium.
PDA is the most widely used medium for growing fungi and bacteria. Due to its semisynthetic composition, variations are possible in each batch. Therefore, a synthetic medium was also selected to conduct the experiments. To this minimal medium (MM), based on the Czapek-Dox recipe by Leslie and Summerel (2006) , 20 g of sucrose and 2 g NaNO3 was added per litre medium. For the experiments conducted on solid media, 15 g agar (Bacteriological Agar Type E, Biokar Diagnostics, Beauvais Cedex, France) per litre was added to the different minimal media. PDA was used as the reference medium.
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