P gsk 3 beta s9

Manufactured by Cell Signaling Technology

Sourced in United States

P-GSK-3-beta (S9) is an antibody that detects phosphorylation of glycogen synthase kinase-3 beta (GSK-3 beta) at serine 9. GSK-3 beta is a serine/threonine protein kinase involved in the regulation of various cellular processes.

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Lab products found in correlation

2 protocols using p gsk 3 beta s9

Signaling Pathway Antibody Panel

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Antibodies against AKT1, p-AKT (S473), p-AKT (T308), p-MEK1/2 (S217/221), MEK1, p-ERK1/2 (p44/42 MAP kinase (phosphorylated Erk1/2)), ERK1/2 (p44/42 MAPK (Erk1/2)), PI3 Kinase p85, p-PTEN (S380/T382/T383), p-Bad (S136), Bad, p-GSK-3-beta (S9) and GSK-3-beta XP were obtained from Cell Signaling Technology; Antibodies against CSGlcA-T, Ki67, pan Phospho-(Ser/Thr) and Heavy chain-free anti-mouse IgG (anti-mouse IgG VeriBlot for IP secondary antibody (HRP)) were obtained from Abcam; Antibodies against PTEN, CK2 and HA-probe were from Santa Cruz Biotechnology; Antibody against Chondroitin-4-sulfate was from Millipore; Antibodies against FLAG-probe and beta-action were from Sigma-Aldrich.

Lin R., Xia S., Shan C., Chen D., Liu Y., Gao X., Wang M., Kang H.B., Pan Y., Liu S., Chung Y.R., Abdel-Wahab O., Merghoub T., Rossi M., Kudchadkar R.R., Lawson D.H., Khuri F.R., Lonial S, & Chen J. (2018). Dietary supplement chondroitin-4-sulfate exhibits oncogene-specific pro-tumor effects on BRAF V600E melanoma cells. Molecular cell, 69(6), 923-937.e8.

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Kinase Inhibitor Profiling Protocol

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AZD8055 and AZD6244 were obtained from SelleckChem (Absource Diagnostics GmbH, Munich, Germany). MK-2206 was obtained from AbMole BioScience (Kowloon, Hongkong). Stock solutions with a concentration of 10 mM were prepared and stored at -80 °C. Antibodies against pan AKT, AKT1, AKT2, pAKT (S473), pAKT (T308), mTOR, pmTOR (S2448), pmTOR (S2481), pERK (T202/Y204), ERK, pMEK (S217/221), MEK 1/2, pGSK3-beta (S9), Cyclin D3, 4EBP-1, SKP-2 and pS6 (S240/244) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against p27, PTEN and HSC-70 were purchased from Santa Cruz. Propidium Iodide (PI) was obtained from Sigma (Taufkirchen, Germany).

Ewald F., Nörz D., Grottke A., Bach J., Herzberger C., Hofmann B.T., Nashan B, & Jücker M. (2015). Vertical Targeting of AKT and mTOR as Well as Dual Targeting of AKT and MEK Signaling Is Synergistic in Hepatocellular Carcinoma. Journal of Cancer, 6(12), 1195-1205.

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