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2 protocols using foretinib

1

Antibody-Mediated Protein Analysis in Cancer Research

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Antibodies against ROS1 (#ab108492) and H3K4me3 (#ab8580) were purchased from Abcam (Cambridge, MA, USA). Antibodies against pROS1(Y2274) (#3078), pERK1/2 (#9106), pAKT(S473) (#4051), AKT (#9272), STAT3 (#9139), EZH2 (#5246), and Gli1 (#2534) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibody against pSTAT3(Y705) (#BS4181) was purchased from Bioworld Technology (Louis Park, MN, USA). Antibody against H3K27me3 (#07-449) was purchased from Merck Millipore. Antibodies against ERK1/2 (#M5670) and GAPDH (#G8795) were purchased from Sigma-Aldrich. Antibodies against STAT1 (#sc-346) and pSTAT1 (#sc-8394) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Alexa Flour 700–conjugated goat anti-mouse IgG secondary antibody (#A21036) was purchased from Invitrogen. IRDye800CW-labeled anti-rabbit secondary antibody (#926-32211) was purchased from LI-COR Biosciences (Lincoln, NE, USA). LY294002 and U0126 were purchased from Calbiochem (San Diego, CA, USA). Foretinib and gefitinib were purchased from ApexBio Technology (Houston, TX, USA). Crizotinib was purchased from Selleckchem (Huston, TX, USA).
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2

Antibody Validation for Immunoblotting and Immunofluorescence

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Anti-ROS1 (#3266, 1:1000 for immunoblotting, 1:100 for immunofluorescence staining) and anti-DRP1 (#8570, 1:1000 for immunoblotting) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-GAPDH (#G8795, 1:5000 for immunoblotting) and anti-myc tag (#05-724MG, 1:1000 for immunoblotting) antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA). Anti-TOM20 (#sc-11415, 1:1000 for immunoblotting, 1:100 for immunofluorescence staining) and anti-TIM23 (#sc-514463, 1:1000 for immunoblotting) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Secondary antibodies conjugated to Alexa Fluor 700 (#A21036), Alexa Fluor 488 (#A11001), Alexa Fluor 555 (#A21428), or Alexa Fluor 647 (#A21235) were purchased from Invitrogen (Carlsbad, CA, USA) and diluted 1:10,000. Rhodamine phalloidin (#A12379) was purchased from Invitrogen and diluted 1:1000 for immunofluorescence staining. IRDye800CW-labeled anti-rabbit secondary antibody (#926-32211, 1:10,000 for immunoblotting) was purchased from LI-COR Biosciences (Lincoln, NE, USA). Anti-GFP tag (#GTX113617, 1:1000 for immunoblotting) and anti-mCherry (#GTX59788, 1:1000 for immunoblotting) antibodies were purchased from GeneTex (Irvine, CA, USA). Foretinib (#A2974) was purchased from ApexBio Technology (Houston, TX, USA).
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