Bds hypersil c18 reverse phase column

An Agilent 1260 HPLC system (Agilent Technologies Inc., Santa Clara, CA, USA) was used for the detection of carvedilol drug levels, equipped with a quaternary pump (G1311B), an autosampler (G7129A), an automatic thermostatic column compartment, a DAD detector (G1315D), and a computer with Agilent OpenLAB CDS Chemstation Edition for LC&LC/MS Systems (Rev C.01.07) for the analysis of the HPLC data. Carvedilol was separated on a BDS Hypersil C18 reverse-phase column (2.1 × 150 mm; 2.4 um) (Thermo Scientific, Waltham, MA, USA) together with a C18 guard column (10 mm × 2.1, 3 um) (Thermo Scientific) with the column temperature set at 35 °C. The mobile phase was a mixture of acetonitrile:buffer (38:62), the buffer was made of 20 mM ammonium acetate, 0.1% triethylamine, and adjusted to pH 4.5 with phosphoric acid. The flow rate was 0.2 mL/min. Carvedilol was analyzed at the wavelength of 240 nm. One μg/mL of propranolol was used as an internal standard (retention time for carvedilol: 6.4; propranolol: 3.9)

After R-carvedilol was received, chiral HPLC analysis was used to determine the purity and accuracy of R-Carvedilol, with Phenomenex Lux^® 5 µm Cellulose-4 LC Column 250 × 4.6 mm (Part No. 00G-4491-E0, Serial Number H21-389311, Batch No. 5599-0063) (Phenomenex, Torrance, CA, USA). The drug level was detected using an Agilent 1260 HPLC system (Agilent Technologies Inc., Santa Clara, CA, USA), equipped with a quaternary pump (G1311B), an autosampler (G7129A), an automatic thermostatic column compartment, a diode array detector (DAD) detector (G1315D), and a computer with Agilent OpenLAB CDS Chemstation Edition for LC&LC/MS Systems (Rev C.01.07). The drug was separated on a BDS Hypersil C18 reverse-phase column (2.1 × 150 mm; 2.4 um) (Thermo Scientific, Waltham, MA, USA) coupled with a C18 guard column (10 mm × 2.1, 3 um) (Thermo Scientific). The mobile phase was a mixture of acetonitrile:buffer (38:62); the buffer was made of 20 mM ammonium acetate, 0.1% triethylamine, and adjusted to pH 4.5 with phosphoric acid. The flow rate was 0.2 mL/min. R-carvedilol was analyzed at the wavelength of 240 nm. Propranolol (1 µg/mL) was used as an internal standard.