The human PBX1 coding region and PARP1coding region was cloned into the pLVX-IRES-mCherry lentiviral vector (Youbio, China). The 10 μg lentiviral vector was cotransfected with 7.5 μg pMD2.G and 2.5 μg psPAX2 (Addgene) into 293T cells in a 100 mm cell culture plate using Lipofectamine (Invitrogen) 3,000 as transfection reagent. The viral particle were harvested at 48 and 72 h after transfection and concentrated by ultracentrifugation (Millipore, United States ). HF-MSCs were transduced with lentiviral particles encoding PBX1 or PARP1 or both PBX1 and PARP1 in the presence of polybrene (Santa Cruz, United States ) at final concentration of 10 μg/ml.
Plvx ires mcherry lentiviral vector
Manufactured by YouBio
Sourced in China
The PLVX-IRES-mCherry lentiviral vector is a tool for gene expression. It allows the simultaneous expression of a gene of interest and a fluorescent marker (mCherry) from a single transcript. The vector contains an internal ribosome entry site (IRES) that enables the translation of both the gene of interest and the mCherry reporter from the same mRNA. This vector can be used to study gene function and monitor transduced cells.
Automatically generated - may contain errors
2 protocols using plvx ires mcherry lentiviral vector
1
Lentiviral Overexpression of PBX1 and PARP1 in HF-MSCs
2
Overexpression of PBX1 and PARP1 in HF-MSCs
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The overexpression of PBX1, PARP1, and PBX1 + PARP1 in HF-MSCs was achieved as previously described [15 (link), 20 (link), 21 ]. The human PBX1 coding region and PARP1coding region was cloned into the pLVX-IRES-mCherry lentiviral vector (Youbio, China). The 10 μg lentiviral vector was cotransfected with 7.5 μg pMD2.G and 2.5 μg psPAX2 (Addgene) into 293 T cells in a 100 mm cell culture plate using Lipofectamine (Invitrogen) 3,000 as transfection reagent. The viral particle were harvested at 48 and 72 h after transfection and concentrated by ultracentrifugation (Millipore, United States). HF-MSCs were transduced with lentiviral particles encoding PBX1 or PARP1 or both PBX1 and PARP1 in the presence of polybrene (Santa Cruz,United States) at final concentration of 10 μg/ml.
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