Total RNA samples were extracted using TRIzol (Invitrogen) and purified using a RNeasy mini-kit (QIAGEN). Gene expression analysis was performed on a GeneChip platform (Human Genome U133A 2.0 Plus; Affymetrix) following the National Health Research Institutes Microarray Core facility technique support. Gene expression changes were calculated as the ratio of 1.2-fold significant alterations of the experimental groups (CTSS overexpression or si-CTSS treated) compared with each control group. Compared with the gene expression variance within each experimental group, most of the olfactory receptor genes detected were differentially expressed.
Human genome u133a 2.0 plus
Manufactured by Thermo Fisher Scientific
The Human Genome U133A 2.0 Plus is a gene expression microarray from Thermo Fisher Scientific. It is designed to measure the expression levels of over 54,000 human transcript variants, representing approximately 38,500 well-characterized human genes. The array provides comprehensive coverage of the human genome.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using human genome u133a 2.0 plus
1
Comprehensive Transcriptomic Analysis of Olfactory Genes
2
Microarray analysis of gene expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA samples were extracted using TRIzol (Invitrogen) and then purified using an RNeasy mini-kit (QIAGEN). Gene expression analysis was performed on a GeneChip platform (Human Genome U133A 2.0 Plus; Affymetrix) according to the manufacturer’s instructions. The gene expression data were deposited in NCBI’s Gene Expression Omnibus (GEO) and are accessible under accession no. GSE23399 . Differentially expressed genes were selected using Student’s t test or Tukey’s honestly significant difference test (P < 0.05; fold-change > 2×). The genes were functionally annotated using DAVID, and the enriched functional gene categories identified were further processed and displayed using Cytoscape. Hierarchical clustering of the selected genes was performed using Cluster and TreeView software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!