Primary antibodies included rat anti-GFAP (Invitrogen, CA); α-tubulin (Sigma-Aldrich, St. Louis, MO, USA), Estrogen Receptor α (C1355) (Millipore, Temecula, CA), Estrogen Receptor β, clone 68-4 (Millipore), S100A4 (Abcam), Pan-cytokeratin MNF116 (Dakocytomation, Denmark); Phospho-EGF Receptor (Tyr1068) and total EGFR (Cell Signaling, Boston, MA). For western blot, secondary antibodies were Alexa-fluor 680 Anti-Rabbit or Anti-Mouse IgG (Invitrogen, NY) detected using an Odyssey Infrared Imaging System (Licor Biosciences, Lincoln, NE). For immunohistochemistry, secondary antibodies were anti-mouse Alexafluor-488 or anti-rabbit Alexafluo-555 (Invitrogen). ER antagonists included 4-OH-tamoxifen (Sigma-Aldrich) and ICI 182780 (Tocris bioscience; R&D systems, Minneapolis, MN). The EGFR/HER2 inhibitor Lapatinib was purchased from Selleckchem (Houston, TX).
Pan cytokeratin mnf116
Manufactured by Agilent Technologies
Sourced in Denmark
Pan-cytokeratin MNF116 is an antibody that recognizes a common epitope shared by several cytokeratin (CK) polypeptides. It is used to detect the presence of various cytokeratins in tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Lapatinib, by Selleck Chemicals (2 mentions)
Ici 182 780, by Bio-Techne (2 mentions)
α tubulin, by Merck Group (2 mentions)
4 oh tamoxifen, by Merck Group (2 mentions)
Phospho egf receptor tyr1068, by Cell Signaling Technology (2 mentions)
Total egfr, by Cell Signaling Technology (2 mentions)
Rat anti gfap, by Thermo Fisher Scientific (2 mentions)
Estrogen receptor α c1355, by Merck Group (2 mentions)
Alexa fluor 680 anti rabbit or anti mouse igg, by Thermo Fisher Scientific (2 mentions)
Anti rabbit alexafluo 555, by Thermo Fisher Scientific (2 mentions)
Anti mouse alexa fluor 488, by Thermo Fisher Scientific (2 mentions)
Odyssey infrared imaging system, by LI COR (2 mentions)
2 protocols using pan cytokeratin mnf116
1
Breast Cancer Molecular Profiling Protocol
2
Breast Cancer Molecular Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Primary antibodies included rat anti-GFAP (Invitrogen, CA); α-tubulin (Sigma-Aldrich, St. Louis, MO, USA), Estrogen Receptor α (C1355) (Millipore, Temecula, CA), Estrogen Receptor β, clone 68-4 (Millipore), S100A4 (Abcam), Pan-cytokeratin MNF116 (Dakocytomation, Denmark); Phospho-EGF Receptor (Tyr1068) and total EGFR (Cell Signaling, Boston, MA). For western blot, secondary antibodies were Alexa-fluor 680 Anti-Rabbit or Anti-Mouse IgG (Invitrogen, NY) detected using an Odyssey Infrared Imaging System (Licor Biosciences, Lincoln, NE). For immunohistochemistry, secondary antibodies were anti-mouse Alexafluor-488 or anti-rabbit Alexafluo-555 (Invitrogen). ER antagonists included 4-OH-tamoxifen (Sigma-Aldrich) and ICI 182780 (Tocris bioscience; R&D systems, Minneapolis, MN). The EGFR/HER2 inhibitor Lapatinib was purchased from Selleckchem (Houston, TX).
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