The 18 residues N-terminal portion of birtoxin-like peptides ‘NH2-ADVPGNYPLD KDGNTYKC’ was commercially synthesized by Sigma and polyclonal antibodies against this peptide were raised by Sigma-Genosys (Inceoglu et al. 2006 (link)). Briefly, the synthetic peptide was cross-linked to keyhole limpet hemocyanin and rabbits were immunized. The bleedings were done after the 4th, 5th and 6th booster doses and pooled. IgG molecules were purified using a Protein A antibody purification kit from Sigma following the manufacturer’s instructions. Protein concentrations were determined using a BCA kit (Pierce, USA) with ovalbumin as the standard.
Protein a antibody purification kit
Manufactured by Merck Group
The Protein A antibody purification kit is a lab equipment product designed for the purification of antibodies from various samples. It utilizes Protein A, a bacterial-derived protein with a high affinity for the Fc region of antibodies, to selectively capture and isolate antibodies from complex mixtures. The core function of this kit is to enable the efficient and specific purification of antibodies for further analysis and applications.
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Lab products found in correlation
2 protocols using protein a antibody purification kit
1
Polyclonal Antibody Production against Birtoxin
2
Protein A-based IgG Antibody Purification
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After filtration of serum through a 0.45 μm membrane, the procedure accompanying the Protein A Antibody Purification Kit (PURE1A, Sigma-Aldrich) was followed in order to obtain human IgG antibodies in solution at physiological pH. IgG antibodies were stored at 4 °C.
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