Bgc823

The GC cell lines including BGC823, HGC27, MKN45 and MGC803 (Type Culture Collection of the Chinese Academy of Sciences) were maintained in Dulbecco's Modified Eagle's medium (GE Healthcare Life Sciences, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) in a 5% CO₂ humidified incubator at 37°C. Cell line characterization was performed by short tandem repeat analysis using GeneMapper^® software (version 4.0; http://www.atcc.org/) and consolidated using the American Type Culture Collection, German Collection of Microorganisms and Cell Cultures, Japanese Collection of Research Bioresources Cell Bank and Rikagaku Kenkyusho (Institute of Physical and Chemical Research, Japan) databases (https://www.atcc.org/en/Products/Cells_and_Microorganisms/Cell_Lines.aspx; https://www.dsmz.de/; http://cellbank.nibiohn.go.jp/english/). The cell lines were tested for the presence of Mycoplasma using PCR (32 (link)).

All cell lines (AGS, BGC-823, HGC-27, SGC-7901, MGC-803, and GES-1) were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). SGC-7901 and BGC-823 cells were cultured in RPMI-1640 medium (GE Healthcare Life Sciences, Logan, UT, USA). AGS, MGC-803, HGC-27, and the normal gastric epithelium cell line GES-1 were cultured in DMEM (GE Healthcare Life Sciences). All the human gastric cancer cell lines and GES-1 cells were supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 100 U/ml penicillin, and 100 mg/ml streptomycin (Invitrogen; Thermo Fisher Scientific, Inc.) and incubated at 37°C under 5% CO₂.