Elan 6000 drc e mass spectrometer

The concentrations (in mg of element per kg of seed) of B, Na, Mg, Al, P, S, K, Ca, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Rb, Sr, Mo, and Cd in seed were measured through ionomics analysis. Approximately 50 mg of seeds was weighed for each sample (Data S1). Three samples were weighed from each panicle. Ionomic analysis was performed using inductively coupled plasma mass spectrometry by the ionomics facility at the Donald Danforth Plant Science Center, as described in Pauli et al. (2018 (link)). Briefly, each seed sample was digested overnight at room temperature in 2.5‐ml nitric acid with 20‐ppb indium as a sample preparation standard. The next day, samples were heated to 100°C for 3 h and then diluted to 10 ml with ultrapure water. Samples were then diluted 5× with ultrapure water containing 2‐ppb yttrium as an instrument standard, using an inline ESI PrepFAST auto dilution system. Element concentrations were measured in a PerkinElmer ELAN 6000 DRC‐e mass spectrometer. Element concentrations obtained from the instrument were then corrected for run‐to‐run variation with the yttrium and indium standards, and a matrix matched control, which was run every 10 samples.