Rat osteocalcin elisa kit

The bone formation marker Osteocalcin (OC) was determined using a rat osteocalcin ELISA kit (Cusabio). The bone resorption markers, such as C-terminal telopeptide fragment of type I collagen (CTX-1; Cusabio), deoxypyridinoline (DPD; Cusabio), and pyridinoline (PYD; MyBioSource, San Diego, USA), were determined using commercial ELISA kits.

Blood samples were centrifuged at 3,000 rpm and 4°C for 15 min; serum samples were collected and stored at −70°C until analysis. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total cholesterol (T-Chol), triglyceride (TG), low-density-lipoprotein cholesterol (LDL-C), and high-density-lipoprotein cholesterol (HDL-C) were measured using an automatic blood chemistry analyzer (BS-220, Mindray, Xuzhou City, China). Measurements of osteocalcin, C-telopeptide of type I collagen (CTX-1), superoxide dismutase (SOD), malonyl dehydrogenase (MDA), endothelin-1, and nitric oxide in serum were performed using the Rat Osteocalcin ELISA kit (Cusabio Biotech, Houston, TX, USA), RatLaps (CTX-1) EIA Assay (Immunodiagnostic System, Gaithersburg, MD, USA), OxiSelect™ Superoxide Dismutase Activity (Cell Biolabs, Inc., San Diego, CA, USA), OxiSelect™ TBARS (Thiobarbituric Acid Reactive Substances) Assay Kit (MDA Quantitation, Cell Biolabs Inc., San Diego, CA, USA), Quantikine ELISA Endothelin-1 kit (R&D Systems, Minneapolis, USA), and colorimetric NO assay kit (R&D Systems, Minneapolis, MN, USA), following the manufacturer's instructions. All samples were analyzed in duplicate in the assay.