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Winglow software mikrowin v4

Manufactured by Berthold Technologies
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WinGlow Software (MikroWin v4.41) is a software application developed by Berthold Technologies. It provides a platform for data acquisition and analysis from various laboratory equipment. The software is compatible with the company's line of instrumentation and enables users to control, monitor, and process data generated by the connected devices.

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Lab products found in correlation

Opti mem medium, by Thermo Fisher Scientific (2 mentions) Bright glo luciferase assay system, by Promega (2 mentions) Chir99021, by Thermo Fisher Scientific (2 mentions) Luminescence plate reader, by Berthold Technologies (2 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (2 mentions)

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WinGlow software (4 citations)

2 protocols using winglow software mikrowin v4

1

Quantifying Wnt/β-catenin Signaling in Cells

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Wnt/β-catenin signaling was measured as previously described 37 . In short, M50 Super 8x TOPflash and M51 Super 8x FOPflash plasmids 70 , containing a firefly luciferase gene under the control of TCF/LEF binding sites (TOPflash) or mutated TCF/LEF binding sites (FOPflash) were used. MLE 12 cells were plated in 48-well plates at a density of 55.000 cells per well. The following day cells were transfected with either 75 ng/well of M50 Super 8x TOPflash plasmid or the negative control M51 Super 8x FOPflash using Lipofectamine 2000 reagent (Life Technologies, Carlsbad, USA) in serum-free Opti-MEM medium (Life Technologies, Carlsbad, USA). After 6 hours of transfection, cells were stimulated for 24h with an agonistic antibody to LTβR [2 μg/ml] (clone ACH6, kindly supplied by Jeffrey Browing, Biogen Idec), recombinant murine TNF-α [1 ng/ml] (Cat. No. 315–01A, PeproTech) and CHIR99021 [1μM] (Cat. No. 4423, Tocris, Minneapolis, MN). Cells were lysed using Glo lysis buffer and luciferase activity was assayed using the Bright-Glo luciferase assay system (Promega, Madison, Wisconsin, USA). Luciferase activity was determined using a luminescence plate reader (Berthold Technologies). Measured values were analyzed with WinGlow Software (MikroWin v4.41, Berthold Technologies) and TOPflash activity was normalized to FOPflash activity and expressed relative to control conditions.
Conlon T.M., John-Schuster G., Heide D., Pfister D., Lehmann M., Hu Y., Ertüz Z., Lopez M.A., Ansari M., Strunz M., Mayr C., Ciminieri C., Costa R., Kohlhepp M.S., Guillot A., Günes G., Jeridi A., Funk M.C., Beroshvili G., Prokosch S., Hetzer J., Verleden S.E., Alsafadi H., Lindner M., Burgstaller G., Becker L., Irmler M., Dudek M., Janzen J., Goffin E., Gosens R., Knolle P., Pirotte B., Stoeger T., Beckers J., Wagner D., Singh I., Theis F.J., de Angelis M.H., O’Connor T.O., Tacke F., Boutros M., Dejardin E., Eickelberg O., Schiller H.B., Königshoff M., Heikenwalder M, & Yildirim A.Ö. (2020). Inhibition of LTβR-signalling activates Wnt-induced regeneration in lung. Nature, 588(7836), 151-156.
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2

Quantifying Wnt/β-catenin Signaling in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wnt/β-catenin signaling was measured as previously described 37 . In short, M50 Super 8x TOPflash and M51 Super 8x FOPflash plasmids 70 , containing a firefly luciferase gene under the control of TCF/LEF binding sites (TOPflash) or mutated TCF/LEF binding sites (FOPflash) were used. MLE 12 cells were plated in 48-well plates at a density of 55.000 cells per well. The following day cells were transfected with either 75 ng/well of M50 Super 8x TOPflash plasmid or the negative control M51 Super 8x FOPflash using Lipofectamine 2000 reagent (Life Technologies, Carlsbad, USA) in serum-free Opti-MEM medium (Life Technologies, Carlsbad, USA). After 6 hours of transfection, cells were stimulated for 24h with an agonistic antibody to LTβR [2 μg/ml] (clone ACH6, kindly supplied by Jeffrey Browing, Biogen Idec), recombinant murine TNF-α [1 ng/ml] (Cat. No. 315–01A, PeproTech) and CHIR99021 [1μM] (Cat. No. 4423, Tocris, Minneapolis, MN). Cells were lysed using Glo lysis buffer and luciferase activity was assayed using the Bright-Glo luciferase assay system (Promega, Madison, Wisconsin, USA). Luciferase activity was determined using a luminescence plate reader (Berthold Technologies). Measured values were analyzed with WinGlow Software (MikroWin v4.41, Berthold Technologies) and TOPflash activity was normalized to FOPflash activity and expressed relative to control conditions.
Conlon T.M., John-Schuster G., Heide D., Pfister D., Lehmann M., Hu Y., Ertüz Z., Lopez M.A., Ansari M., Strunz M., Mayr C., Ciminieri C., Costa R., Kohlhepp M.S., Guillot A., Günes G., Jeridi A., Funk M.C., Beroshvili G., Prokosch S., Hetzer J., Verleden S.E., Alsafadi H., Lindner M., Burgstaller G., Becker L., Irmler M., Dudek M., Janzen J., Goffin E., Gosens R., Knolle P., Pirotte B., Stoeger T., Beckers J., Wagner D., Singh I., Theis F.J., de Angelis M.H., O’Connor T.O., Tacke F., Boutros M., Dejardin E., Eickelberg O., Schiller H.B., Königshoff M., Heikenwalder M, & Yildirim A.Ö. (2020). Inhibition of LTβR-signalling activates Wnt-induced regeneration in lung. Nature, 588(7836), 151-156.
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