Proteins were separated on 10% SDS-PAGE and then transferred to 0.45 μm PVDF membranes (Amersham, UK). The membranes were incubated with 5% non-fat dried milk overnight at 4°C and with anti-KRAS antibody (CST, USA) at 1:1000 dilution for 2h at room temperature, anti-Bad/p/Bad antibody (Bio-world, USA) at 1:500 dilution, anti-AKT/p-AKT antibody (CST, USA) at 1:1000 dilution and anti-GAPDH antibody (Proteintech, USA) at 1:30,000 dilution. After washing twice with TBST, the membranes were incubated with goat anti-rabbit antibody (zsgb-bio, China) at 1:5000 and 1:50000 dilutions for 2 h.
0.45 μm pvdf membranes
Manufactured by Cytiva
Sourced in United Kingdom
The 0.45 μm PVDF membranes are a type of lab equipment used for filtration purposes. They are made of polyvinylidene fluoride (PVDF) material and have a pore size of 0.45 micrometers.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 0.45 μm pvdf membranes
1
Western Blot Analysis of Key Proteins
2
Western Blot Analysis of Cell Signaling Proteins
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Proteins were separated on 10% SDS-PAGE and then transferred to 0.45 μm PVDF membranes (Amersham, UK). The membranes were incubated with 5% non-fat dried milk overnight at 4°C and with anti-AKT2 monoclonal antibody (Proteintech, USA) at 1:1000 dilution; anti-Bad monoclonal antibody (Bioworld, USA) at 1:500 dilution; anti-GSK-3B polyclonal antibody (Bioworld, USA) at 1:1000 dilution; anti-GAPDH antibody (Proteintech, Chicago, USA) at 1:30,000 dilution. After washing twice with TBST, the membranes were incubated with goat anti-rabbit antibody (zsgb-bio, China) at 1:5000 and 1:50000 dilutions for 2 h.
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