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Rhodamine and alexa fluor 488 labelled phalloidin

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Rhodamine and Alexa Fluor 488-labelled phalloidin are fluorescent probes used in microscopy and flow cytometry applications. Phalloidin is a toxin that binds specifically to F-actin, allowing the visualization of the actin cytoskeleton. The Rhodamine and Alexa Fluor 488 labels provide different excitation and emission spectra, enabling their detection using different fluorescence channels.

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Lab products found in correlation

Formamide, by Merck Group (2 mentions) Anti ve cadherin ab33168, by Abcam (2 mentions) Anti ve cadherin f 8, by Santa Cruz Biotechnology (2 mentions) Anti trio h 120, by Santa Cruz Biotechnology (2 mentions) Anti notch1 icd c 20 r, by Santa Cruz Biotechnology (2 mentions) Anti notch1 ecd h 131, by Santa Cruz Biotechnology (2 mentions) Anti ve ptp h 300, by Santa Cruz Biotechnology (2 mentions) Anti rfp ab124754, by Abcam (2 mentions) Af647 conjugate if, by Cell Signaling Technology (2 mentions) Anti rac1 antibody 102, by BD (2 mentions) Anti ptprf antibody sab4200321, by Merck Group (2 mentions) Dynasore hydrate, by Merck Group (2 mentions) Anti β tubulin ab6046, by Abcam (2 mentions) Rac1 inhibitor nsc23766, by Santa Cruz Biotechnology (2 mentions) Evans blue dye, by Merck Group (2 mentions)

2 protocols using rhodamine and alexa fluor 488 labelled phalloidin

1

Comprehensive Immunostaining Protocol for Endothelial Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
The anti-Trio (H-120, 1:500), anti-Notch1 ICD (C-20-R, 1:1000), anti-Notch1 ECD (H-131, 1:500), anti-VE-cadherin (F-8, 1:200), and anti-VE-PTP (H-300, 1:250) antibodies were from Santa Cruz Biotechnology. Anti-VE-cadherin (ab33168, 1:2,000), anti-RFP (ab124754, 1:1000), and anti-β tubulin (ab6046, 1:5,000) antibodies were from Abcam. Anti-VEGFR2 antibody (55B11, 1:1000), anti-Notch1 V1754G (D3B8, 1:1000 WB), anti-HA (6E2, 1:1000 WB, AF647 conjugate IF) was from Cell Signaling Technologies. Anti-Rac1 antibody (102, 1:1,000) was from BD Biosciences. Rhodamine and Alexa Fluor 488-labelled phalloidin, Alexa Fluor-488, 568 and 647 goat anti-mouse and anti-rabbit IgG secondary antibodies were from Life Technologies. HRP-conjugated donkey anti-mouse, anti-rabbit, and anti-goat IgG secondary antibodies were from Fitzgerald. Anti-PTPRF antibody (SAB4200321, 1:1000), DAPI, DAPT, Evans Blue dye, dynasore hydrate, and formamide were from Sigma. Rac1 inhibitor NSC 23766 was from Santa Cruz Biotechnology.
Polacheck W.J., Kutys M.L., Yang J., Eyckmans J., Wu Y., Vasavada H., Hirschi K.K, & Chen C.S. (2017). A non-canonical Notch complex regulates adherens junctions and vascular barrier function. Nature, 552(7684), 258-262.
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2

Comprehensive Immunostaining Protocol for Endothelial Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
The anti-Trio (H-120, 1:500), anti-Notch1 ICD (C-20-R, 1:1000), anti-Notch1 ECD (H-131, 1:500), anti-VE-cadherin (F-8, 1:200), and anti-VE-PTP (H-300, 1:250) antibodies were from Santa Cruz Biotechnology. Anti-VE-cadherin (ab33168, 1:2,000), anti-RFP (ab124754, 1:1000), and anti-β tubulin (ab6046, 1:5,000) antibodies were from Abcam. Anti-VEGFR2 antibody (55B11, 1:1000), anti-Notch1 V1754G (D3B8, 1:1000 WB), anti-HA (6E2, 1:1000 WB, AF647 conjugate IF) was from Cell Signaling Technologies. Anti-Rac1 antibody (102, 1:1,000) was from BD Biosciences. Rhodamine and Alexa Fluor 488-labelled phalloidin, Alexa Fluor-488, 568 and 647 goat anti-mouse and anti-rabbit IgG secondary antibodies were from Life Technologies. HRP-conjugated donkey anti-mouse, anti-rabbit, and anti-goat IgG secondary antibodies were from Fitzgerald. Anti-PTPRF antibody (SAB4200321, 1:1000), DAPI, DAPT, Evans Blue dye, dynasore hydrate, and formamide were from Sigma. Rac1 inhibitor NSC 23766 was from Santa Cruz Biotechnology.
Polacheck W.J., Kutys M.L., Yang J., Eyckmans J., Wu Y., Vasavada H., Hirschi K.K, & Chen C.S. (2017). A non-canonical Notch complex regulates adherens junctions and vascular barrier function. Nature, 552(7684), 258-262.
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