Sc 6465

Manufactured by Santa Cruz Biotechnology

Sourced in United States

SC-6465 is a laboratory centrifuge designed for general-purpose sample processing. It features a compact design and can accommodate a range of rotor sizes to handle different sample volumes. The centrifuge is capable of achieving high-speed rotation to facilitate efficient separation of components within liquid samples.

Automatically generated - may contain errors

Lab products found in correlation

Ab2728, by Abcam (1 mentions) Ab55667, by Abcam (1 mentions) Sc 15363, by Santa Cruz Biotechnology (1 mentions) Alexa flour 488 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) A21206, by Thermo Fisher Scientific (1 mentions) A21202, by Thermo Fisher Scientific (1 mentions) A11073, by Thermo Fisher Scientific (1 mentions) A11055, by Thermo Fisher Scientific (1 mentions) A11058, by Thermo Fisher Scientific (1 mentions) C6198, by Merck Group (1 mentions) A28175, by Thermo Fisher Scientific (1 mentions) A8273, by Merck Group (1 mentions)

2 protocols using sc 6465

Isolation and Analysis of Hepatoma Cell-Derived Extracellular Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Huh-7, Hep G2 human hepatoma cell lines and Hepa 1–6 mouse hepatoma cell line were purchased from American Type Culture Collection (ATCC; Rockville, MD, USA). Human IRF-1 expression plasmid and backbone plasmid were ordered from Origene (Rockville, MD). β-galactosidase expression (β-Gal) plasmid was purchased from Promega (Madison, WI). Mouse IRF-1 expression adenovirus (Ad-mIRF-1) and β-galactosidase expression adenovirus (Ad-LacZ), mice Ad-Rab27a shRNA, adenovirus scramble shRNA (Ad-SCR) and control adenovirus backbone (Ad-CTL), targeting both human and mice Ad-IRF-1 shRNA were ordered from Vector Biolabs (Malvern, PA). Antibodies against IRF-1 (SC-640, Santa Cruz), CD63 (SC-15363, Santa Cruz), calnexin (SC-6465, Santa Cruz), CD81 (160523-006, System Biosciences), HSP70 (ab2728, Abcam), Rab27a (ab55667, Abcam), β-actin (8H10D10, CST), Lamin A/C (4C11, CST) were used for Western Blot. TopFluor™ labeled E06 antibody (Avanti Polar Lipids), which targets oxidized phospholipid, was used to detect oxidized phospholipid on EVs by flow cytometry.

Yang M.Q., Du Q., Goswami J., Varley P.R., Chen B., Wang R.H., Morelli A.E., Stolz D.B., Billiar T.R., Li J, & Geller D.A. (2018). Interferon regulatory factor 1-Rab27a regulated extracellular vesicles promote liver ischemia/reperfusion injury. Hepatology (Baltimore, Md.), 67(3), 1056-1070.

+ Open protocol

+ Expand

Comprehensive Antibody Labeling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following primary antibodies were used: Guinea pig anti-CAPS2 [1:100 (Sadakata et al., 2004 (link); Sadakata et al., 2006 (link))], rabbit anti-α-amylase (1:200; A8273, Sigma-Aldrich, Saint Louis, MO, United States), mouse anti-actin, α-smooth muscle-Cy3 (1:500; C6198, Sigma-Aldrich), goat anti-calnexin (1:100; sc-6465, Santa Cruz Biotechnology, Santa Cruz, CA, United States), mouse anti-GM130 (1:150; 610822, BD Transduction Laboratories, San Jose, CA, United States), mouse APC anti-mouse CD3ε (1:300; 100312, BioLegend Inc., San Diego, CA, United States), and rabbit anti-TGN38 (1:100; T9826, Sigma-Aldrich). The secondary antibodies used were: Alexa Flour 488 goat anti-guinea pig IgG (H + L) (1:500; A11073, Invitrogen, Carlsbad, CA, United States), Alexa Flour 488 goat anti-mouse IgG (H + L) (1:1,000; A28175, Invitrogen), Alexa Flour 488 donkey anti-rabbit IgG (H + L) (1:5,000; A21206, Invitrogen), Alexa Flour 488 donkey anti-mouse IgG (H + L) (1:1,000; A21202, Invitrogen), Alexa Flour 488 donkey anti-mouse IgG (H + L) (1:1,000; A21202, Invitrogen), Alexa Flour 546 donkey anti-goat IgG (H + L) (1:1,000; A11058, Invitrogen), Alexa Fluro555 donkey anti-goat IgG (H + L) (1:1,000; A11055, Invitrogen), and Alexa Flour 594 donkey anti-goat IgG (H + L) (1:1,000; A11058, Invitrogen).

Sato Y., Tsuyusaki M., Takahashi-Iwanaga H., Fujisawa R., Masamune A., Hamada S., Matsumoto R., Tanaka Y., Kakuta Y., Yamaguchi-Kabata Y., Furuse T., Wakana S., Shimura T., Kobayashi R., Shinoda Y., Goitsuka R., Maezawa S., Sadakata T., Sano Y, & Furuichi T. (2022). Loss of CAPS2/Cadps2 leads to exocrine pancreatic cell injury and intracellular accumulation of secretory granules in mice. Frontiers in Molecular Biosciences, 9, 1040237.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!