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Fluorokine e kit

Manufactured by R&D Systems
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Sourced in United Kingdom

The Fluorokine E kit is a laboratory tool used for the detection and quantification of a specific analyte in a sample. It utilizes fluorescence-based detection technology to provide accurate and sensitive measurements. The kit includes the necessary reagents and materials to perform the analysis.

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Lab products found in correlation

Cytometric bead array human th1 th2 th17 cytokine kit, by BD (1 mentions) Accuri c6 flow cytometer, by BD (1 mentions) Quantikine enzyme linked immunoassay kit, by R&D Systems (1 mentions) Fluorometric assay kit, by Abcam (1 mentions) Mmp 9, by R&D Systems (1 mentions)

Spelling variants of this product

Human Active MMP-1 Fluorokine E kit (7 citations) Human Active MMP-9 Fluorokine E kit (7 citations) Fluorokine® E human active MMP-1 fluorescent assay kit (5 citations)

3 protocols using fluorokine e kit

1

Multiplex Cytokine Analysis Protocol

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Serum IFN-γ levels were measured using the BD™ Cytometric Bead Array Human Th1/Th2/Th17 cytokine kit (Becton, Dickinson and Company, Franklin Lakes, NJ) via the BD Accuri C6 flow cytometer with a sequential multi-channel analyzer (Becton Dickinson). Serum periostin was measured using an enzyme-linked immunoassay kit (Phoenix Pharmaceuticals, Burlingame, CA). MMP-1 was measured using the Fluorokine E kit (R&D Systems, Minneapolis, MN) and MMP-9 using the Quantikine enzyme-linked immunoassay kit (R&D Systems). All assays were performed according to the manufacturers’ instructions.
Yamauchi M., Kinjo T., Parrott G., Miyagi K., Haranaga S., Nakayama Y., Chibana K., Fujita K., Nakamoto A., Higa F., Owan I., Yonemoto K, & Fujita J. (2020). Diagnostic performance of serum interferon gamma, matrix metalloproteinases, and periostin measurements for pulmonary tuberculosis in Japanese patients with pneumonia. PLoS ONE, 15(1), e0227636.
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2

Quantification of MMP-9, PE, and LTA4H in BAL Fluid

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The concentration of MMP-9 (R&D Systems, Minneapolis, MN), PE (MyBiosource, San Diego, CA, USA) and LTA4H (USCN Life Science, Hubei, PRC) in BAL fluid was measured using an ELISA, according to the manufacturer's directions. BAL fluid MMP-9 activity was determined by Fluorokine E kit (R&D Systems, Minneapolis, MN), and NE activity by a Fluorometric assay kit (Abcam, Cambridge, UK), according to the manufacturer's directions.
Turnbull A.R., Pyle C.J., Patel D.F., Jackson P.L., Hilliard T.N., Regamey N., Tan H.L., Brown S., Thursfield R., Short C., Mc Fie M., Alton E.W., Gaggar A., Blalock J.E., Lloyd C.M., Bush A., Davies J.C, & Snelgrove R.J. (2020). Abnormal pro-gly-pro pathway and airway neutrophilia in pediatric cystic fibrosis. Journal of Cystic Fibrosis, 19(1), 40-48.
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3

Quantitative Analysis of MMP13 Activity

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MMP13 activity was analyzed using the Fluorokine E kit (R&D) following the manufacturer’s instructions. When 35S labeling was performed supernatant was collected just prior to labeling.
Katsara O., Attur M., Ruoff R., Abramson S.B, & Kolupaeva V. (2017). Increased activity of chondrocyte translational apparatus accompanies osteoarthritis. Arthritis & rheumatology (Hoboken, N.J.), 69(3), 586-597.
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