The fractional exhaled nitric oxide (FENO) level was measured using Hypair FeNO+ Device (Medisoft; Sorinnes, Belgium) with expiratory airflow of 50, 100, 150, and 350 ml/s. FENO concentrations were classified following the American Thoracic Society/European Respiratory Society (ATS/ERS) recommendation for children: <20 ppb: normal; 20–35 ppb: increased; and >35 ppb: highly enriched (23 (link)).
Hypair feno device
Manufactured by Medisoft
Sourced in Belgium
The Hypair FeNO+ Device is a portable medical device used for the measurement of fractional exhaled nitric oxide (FeNO) levels in the breath. FeNO is a biomarker that can provide information about airway inflammation, which is often associated with conditions like asthma. The device is designed to accurately and conveniently measure FeNO levels, providing healthcare professionals with valuable data for patient assessment and management.
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4 protocols using hypair feno device
1
Fractional Exhaled Nitric Oxide Measurement
2
Measuring Nasal and Airway Nitric Oxide
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Nasal exhaled NO (nasal FENO) was measured with constant aspiratory flow using the Hypair FeNO+ Device (Medisoft), which is an electrochemical based analyzer. The procedure is as follows: 1) patient connects a catheter to one of his/her nostrils; 2) air from the nasal cavity is continuously analyzed by the NO electrochemical sensor; and 3) throughout the analysis, patient breathes through an expiratory brake, so that the velum is closed to prevent any contamination of nasal air with bronchial air. The aspiration flow rate was 100 mL/second through a nasal catheter.
For the airways, bronchial FENO and alveolar concentration of NO (CANO) were measured at multiple flow rates (50, 100, 150, and 350 mL/second). Technical measurement of exhaled NO was taken according to manufacturer’s instructions, as recommended by the American Thoracic Society/European Respiratory Society guideline and the results of exhaled NO had been reported by Hypair FeNO+ Expair Software (Medisoft).15
For the airways, bronchial FENO and alveolar concentration of NO (CANO) were measured at multiple flow rates (50, 100, 150, and 350 mL/second). Technical measurement of exhaled NO was taken according to manufacturer’s instructions, as recommended by the American Thoracic Society/European Respiratory Society guideline and the results of exhaled NO had been reported by Hypair FeNO+ Expair Software (Medisoft).15
3
Measurement of Exhaled Nitric Oxide
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Measurement of the concentration of nitric oxide exhaled with air from the bronchi (FeNO) was performed using the HypAir FeNO device by Medisoft. At least two FeNO measurements were performed in each patient. The mean value of the measurements was taken as the final result. The examination was conducted and its results interpreted in accordance with the recommendations of the American Thoracic Society (ATS) [25 (link), 26 (link)].
4
Evaluating Lung Function and Exhaled NO
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LFT (spirometry) was done by Koko (nSpire Health, Inc., Longmont, CO, USA). The reversibility of forced expiratory volume in one second (FEV1) was evaluated after using 200 μg of salbutamol for 15 min. The test was positive when there was the increase of FEV1 ≥12% and >200 mL (14 (link)). Measuring exhaled NO concentration was done by Hypair FeNO+ Device (Medisoft; Sorinnes, Belgium) with expiratory air flow of 50, 100, 150, and 350 mL/s. The fractional exhaled nitric oxide (FENO) levels were classified as recommended by the American Thoracic Society/European Respiratory Society (ATS/ERS) for children (<20 ppb: normal, 20–35 ppb: increased, and >35 ppb: highly increased) (15 (link)). The level of alveolar concentration of exhaled NO (CANO) <5 ppb was defined as normal (15 (link)).
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