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3 iodo l tyrosine 3iy

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3-iodo-L-tyrosine (3IY) is a chemical compound used as a research tool in various scientific applications. It is a derivative of the amino acid tyrosine, with an iodine atom substituted at the 3-position of the aromatic ring. 3IY is commonly employed in biochemical studies, but its specific uses and intended applications are not provided in this factual description.

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Lab products found in correlation

Triton x 100, by Merck Group (1 mentions) Trypsin edta 0.025 solution, by Lonza (1 mentions) L dopa, by Merck Group (1 mentions) S carbidopa, by Merck Group (1 mentions)

Spelling variants of this product

L-tyrosine (227 citations) Tyrosine (137 citations) 3-iodo-L-tyrosine (6 citations)

3 protocols using 3 iodo l tyrosine 3iy

1

3-iodo-L-tyrosine Labeling Protocol

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3-iodo-L-tyrosine (3IY) (≥95%) and Triton X-100 were purchased from Sigma Aldrich. Trypsin-EDTA 0.025% solution was purchased from Lonza. All other chemicals used for PBS X1 preparation NaCl, KCl, Na2HPO4, KH2PO4 and HCl (36%) were of analytical grade. Other chemicals that we used are listed in the in vitro section.
Filošević Vujnović A., Jović K., Pištan E, & Andretić Waldowski R. (2021). Influence of Dopamine on Fluorescent Advanced Glycation End Products Formation Using Drosophila melanogaster. Biomolecules, 11(3), 453.
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2

Dopaminergic Drugs Feeding Assay

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3-Iodo-L-tyrosine (3-IY) (I8250, Sigma) and L-DOPA (D9628, Sigma) were mixed directly into melted fly food at final concentrations of 1.25 mg/ml and 1 mg/ml, respectively. S-(−)-Carbidopa (C1335, Sigma) was dissolved in 1 ml of water at the 10× final concentration and mixed with 9 ml of melted fly food. The Trikinetics activity monitor tubes were loaded with a plug of food at one end, with drug feeding throughout the course of the experiment.
Cichewicz K., Garren E.J., Adiele C., Aso Y., Wang Z., Wu M., Birman S., Rubin G.M, & Hirsh J. (2016). A New Brain Dopamine Deficient Drosophila and its Pharmacological and Genetic Rescue. Genes, brain, and behavior, 16(3), 394-403.
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3

Preparation and Administration of 3IY and L-dopa

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3-iodo-L-tyrosine (3IY) (Sigma-Aldrich, 70-78-0) and L-DOPA-ring-d3 (L-dopa) (Sigma-Aldrich, 53587-29-4) were used. The protocols for administration of 3IY and L-dopa were described previously7 (link). The concentrations of 3IY and L-dopa in yeast paste were 10 mg/ml and 0.5 mg/ml, respectively.
Pu Y., Palombo M.M, & Shen P. (2018). Contribution of DA Signaling to Appetitive Odor Perception in a Drosophila Model. Scientific Reports, 8, 5978.
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