Bax/Bak WT and double-knockout (DKO) MEFs, used in our previous study [41 (link)], RT4-D6P2T (RT4) schwannoma cells [66 (link)], purchased from Sigma (93011415), and HeLa cells were cultured in DMEM (D6046) media with 10% fetal bovine serum (FBS) (12133C) (Sigma).
Fbs 12133c
Manufactured by Merck Group
Sourced in Germany
FBS (12133C) is a laboratory product manufactured by Merck Group. It is a cell culture reagent used to support the growth and maintenance of cells in vitro. The core function of FBS is to provide a source of essential nutrients, growth factors, and other components necessary for cell proliferation and survival.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fbs 12133c
1
Cell Culture of MEFs, Schwannoma, and HeLa
2
Cell Culture and Transfection Protocols for Multiple Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HeLa, HEK293, SK-N-SH, RT4-D6P2T (from Sigma, 93011415), and LAMP1/2 double knock-out or wild-type MEFs (gifts from Dr. P. Saftig, Kiel, Germany) were cultured in DMEM (D6046) medium with 10% FBS (12133C) (Sigma). mRFP-GFP-LC3–stably expressing HeLa cells, described previously (28 (link), 60 (link)), were cultured in DMEM with 10% FBS. Human primary schwannomas were cultured as described previously (63 (link)) with full ethical approval.
Plasmids were transfected into cells either alone or with siRNA using Lipofectamine 2000 (Thermo Scientific), according to the manufacturer's instructions, for the length of time indicated in each figure legend.
Plasmids were transfected into cells either alone or with siRNA using Lipofectamine 2000 (Thermo Scientific), according to the manufacturer's instructions, for the length of time indicated in each figure legend.
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