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Clone dx2

Manufactured by BD
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The Clone Dx2 is a laboratory equipment designed for cloning purposes. It facilitates the replication of DNA samples.

Automatically generated - may contain errors

Lab products found in correlation

Paraformaldehyde (pfa), by Merck Group (1 mentions) Wst 1 assay, by Roche (1 mentions) Clone sp34 2, by BD (1 mentions) Clone cd28, by BD (1 mentions) Aqua amine reactive dye, by Thermo Fisher Scientific (1 mentions) Clone hil 7r m21, by BD (1 mentions) Clone m a251, by BD (1 mentions) Lsr 2 flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Anti-CD95-PE-Cy5 (clone DX2, CD95 APC (clone DX2), CD95-PE-Cy5 (clone DX2), APC anti-CD95 (clone DX2, CD95 (clone DX2,

2 protocols using clone dx2

1

Evaluating miRNA Effects on APAP- and FAS-Induced Hepatocyte Damage

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For APAP-induced hepatocyte damage, hepatocytes were treated with 3 mg ml−1 APAP (Sigma) at 24 h after miRNA transfection. Likewise, for FAS-induced hepatocyte damage, hepatocytes were treated with 1 μg ml−1 Hamster anti-mouse CD95 antibody, clone Jo2 (BD Pharmingen) for (mouse hepatocytes) or mouse anti-human CD95 antibody, clone Dx2 (BD Pharmingen) (for human hepatocytes) at 24 h after miRNA transfection. The cell viability was measured by WST-1 assay (Roche, Switzerland) at 6 h after CD95 or APAP treatment. Absorbance was measured at 440 nm. In vivo ALF was induced by intraperitoneal injection of 350 mg kg−1 APAP (Sigma) in 8- to 10-week-old BALB/c mice. Likewise, for FAS-induced ALF, 0.5 μg  per gram body weight Hamster anti-mouse CD95 antibody (BD Pharmingen) was injected intraperitoneally. Mice were either monitored for survival or killed 6 h after injection. Liver tissues were harvested and immediately snap frozen in liquid nitrogen and fixed in 4% paraformaldehyde (Sigma).
Yang D., Yuan Q., Balakrishnan A., Bantel H., Klusmann J.H., Manns M.P., Ott M., Cantz T, & Sharma A.D. (2016). MicroRNA-125b-5p mimic inhibits acute liver failure. Nature Communications, 7, 11916.
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2

Multiparametric Flow Cytometry of PBMCs

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Freshly-isolated PBMCs (5×105 cells) were surface stained with the viability dye, Aqua Amine Reactive Dye (Invitrogen), as well as monoclonal antibodies directed against CD3 (Clone SP 34-2, BD Biosciences), CD4 (NIH-Nonhuman Primate Reagent Resource Program), CD8 (Clone 3B5, Invitrogen), CD25 (Clone M-A251, BD Biosciences or Clone 4E3, Miltenyi), CD95 (Clone DX2, BD Biosciences) or CD45RA (Clone 2H4, Beckman Coulter), CD27 (Clone M-T271, BD Biosciences) or CD28 (Clone CD28.2, BD Biosciences), and CD127 (Clone hIL-7R-M21, BD Biosciences) for 20 minutes at room temperature, fixed and then permeabilized with Affymetrix FoxP3 Fix/Perm Buffers (Affymetrix) per the manufacturer’s instructions. Permeabilized cells were then stained intracellularly for Ki-67 (Clone B56, BD Biosciences) and FoxP3 (Clone PCH101, Affymetrix) for 30 minutes at 4°C, washed in PBS-2% FBS and analyzed by flow cytometry. A minimum of 150,000 events was acquired on a BD LSRII flow cytometer (BD Biosciences) and analyzed using FlowJo software (Tree Star, Inc.).
Baker C.A., Swainson L., Lin D.L., Wong S., Hartigan-O’Connor D., Lifson J.D., Tarantal A.F, & McCune J.M. (2015). Exposure to SIV In Utero Results in Reduced Viral Loads and Altered Responsiveness to Postnatal Challenge. Science translational medicine, 7(300), 300ra125.
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