Pgcn2 t899

Manufactured by Abcam

Sourced in United States

PGCN2 (T899) is a protein-specific antibody that targets the PGCN2 protein. It is designed for use in various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to detect and analyze the PGCN2 protein in biological samples.

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2 protocols using pgcn2 t899

Antibody Detection of Glucose and Signaling Proteins

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Rabbit antibodies against human GLUT1, GLUT 4, GCN2, pGCN2 (T899) and eIF2α were obtained from Abcam, Cambridge, MA. FASN, pACC (S79), ACC, CPT-1 antibodies were obtained from Cell Signaling Technology, Danvers, MA and mouse antibody against human peIF2α (S52) was obtained from Invitrogen, Grand Island, NY. All the antibodies were used at 1:1000 dilution.

Chattopadhyay N., Berger A.J., Koenig E., Bannerman B., Garnsey J., Bernard H., Hales P., Maldonado Lopez A., Yang Y., Donelan J., Jordan K., Tirrell S., Stringer B., Xia C., Hather G., Galvin K., Manfredi M., Rhodes N, & Amidon B. (2015). KRAS Genotype Correlates with Proteasome Inhibitor Ixazomib Activity in Preclinical In Vivo Models of Colon and Non-Small Cell Lung Cancer: Potential Role of Tumor Metabolism. PLoS ONE, 10(12), e0144825.

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Immunoblot Analysis of Cellular Proteins

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Lysates were prepared and electrophoresed on gels as described previously (32 ). Membranes were probed with primary antibodies following each of the supplier’s recommendations: AKT (#4685), pAKT (S473) (#4060), ATG5 (#2630), caspase-3 (#9662), Cleaved PARP (D214) (#9541), p-eIF2α (S51) (#3398), ERK1/2 (#9102), pERK1/2 (T202/Y204) (#9101), GCN2 (#3302), LC3B (#2775), PRAS40 (#2610), pPRAS40 (T246) (#2997), and p62 (#5114) from Cell Signaling Technology (Danvers, MA), and p21 (sc-756), p27 (sc-528), Cyclin D1 (sc-718), α enolase (sc-7455), VDAC1 (sc-8828), and ERK2 (sc-1647) from Santa Cruz Biotechnology (Dallas, TX, USA). The P5CS primary antibody, ALDH18A1 (NBP1-83324), was purchased from Novus Biologicals (Littleton, CO, USA) and pGCN2 (T899) (ab75836) from Abcam. eIF2α and eIF2Bε antibodies were kind gifts from Dr. Scot Kimball. Secondary antibodies goat anti-rabbit IgG-HRP (sc-2004), goat anti-mouse IgG-HRP (sc-2005), and donkey anti-goat IgG-HRP (sc-2020) were purchased from Santa Cruz Biotechnology. The immunoblots were developed using ECL Western Blotting Substrate (Thermo Scientific) or Supersignal West Femto Chemiluminescent Substrate (Thermo Scientific).

Kardos G.R., Wastyk H.C, & Robertson G.P. (2015). Disruption of Proline Synthesis in Melanoma Inhibits Protein Production Mediated by the GCN2 Pathway. Molecular cancer research : MCR, 13(10), 1408-1420.

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