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Bv650 anti cd45ro clone uchl1

Manufactured by BD
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The BV650 anti-CD45RO (clone UCHL1) is a fluorochrome-conjugated monoclonal antibody that binds to the CD45RO isoform of the human CD45 protein. It is designed for use in flow cytometry applications to identify and characterize T cells expressing the CD45RO marker.

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Lab products found in correlation

Human fcr blocking reagent, by Miltenyi Biotec (2 mentions) Percp cy5.5 anti cd4 clone okt4, by BioLegend (2 mentions) Aqua live dead fixable staining reagent, by Thermo Fisher Scientific (2 mentions) Apc anti cd3 clone okt3, by BioLegend (2 mentions) Pe cy7 anti cd45ra clone hi100, by BioLegend (2 mentions) Pacific blue anti cxcr3 clone g025h7, by BioLegend (2 mentions) Bb515 anti ccr6 clone 11a9, by BD (2 mentions)

Spelling variants of this product

Anti-CD45RO (21 citations) CD45RO (clone UCHL1), (9 citations) CD45RO (UCHL1, (7 citations) Clone UCHL1 (5 citations) Anti-CD45RO (clone UCHL1, (3 citations) UCHL1 (3 citations)

2 protocols using bv650 anti cd45ro clone uchl1

1

Sorting and Expansion of Th1, Th1/Th17, and Th17 Cells from RRMS Patients

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Fresh peripheral blood samples from de-identified RRMS patients were collected under the approvement by the Northwestern University Institutional Review Board. Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using ISOLYMPH (Cat #759050, CTL). The PBMCs were stained with Aqua LIVE/DEAD fixable staining reagents (Cat #L34957, Life Technologies), human FcR blocking reagent (Cat # 130-059-901, Miltenyi Biotec), APC anti-CD3 (clone OKT3, BioLegend), Percp-cy5.5 anti-CD4 (clone OKT4, BioLegend) PE-Cy7 anti-CD45RA (clone HI100, BioLegend), BV650 anti-CD45RO (clone UCHL1, BD Biosciences), Pacific Blue anti-CXCR3 (clone G025H7, BioLegend) and BB515 anti-CCR6 (clone 11A9, BD Biosciences). Then CD3+CD4+CD45RA−CD45RO+CCR6−CXCR3+ (Th1), CD3+CD4+CD45RA−CD45RO+CCR6+CXCR3+ (Th1/Th17) and CD3+CD4+ CD45RA−CD45RO+CCR6+CXCR3− (Th17) cells were sorted. After sorting, the cells were amplified by stimulated with anti-CD3/CD28 Dynabeads for 7 days.
Qian Y., Arellano G., Ifergan I., Lin J., Snowden C., Kim T., Thomas J.J., Law C., Guan T., Balabanov R.D., Kaech S.M., Miller S.D, & Choi J. (2021). ZEB1 promotes pathogenic Th1 and Th17 cell differentiation in multiple sclerosis. Cell reports, 36(8), 109602.
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2

Sorting and Expansion of Th1, Th1/Th17, and Th17 Cells from RRMS Patients

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fresh peripheral blood samples from de-identified RRMS patients were collected under the approvement by the Northwestern University Institutional Review Board. Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using ISOLYMPH (Cat #759050, CTL). The PBMCs were stained with Aqua LIVE/DEAD fixable staining reagents (Cat #L34957, Life Technologies), human FcR blocking reagent (Cat # 130-059-901, Miltenyi Biotec), APC anti-CD3 (clone OKT3, BioLegend), Percp-cy5.5 anti-CD4 (clone OKT4, BioLegend) PE-Cy7 anti-CD45RA (clone HI100, BioLegend), BV650 anti-CD45RO (clone UCHL1, BD Biosciences), Pacific Blue anti-CXCR3 (clone G025H7, BioLegend) and BB515 anti-CCR6 (clone 11A9, BD Biosciences). Then CD3+CD4+CD45RA−CD45RO+CCR6−CXCR3+ (Th1), CD3+CD4+CD45RA−CD45RO+CCR6+CXCR3+ (Th1/Th17) and CD3+CD4+ CD45RA−CD45RO+CCR6+CXCR3− (Th17) cells were sorted. After sorting, the cells were amplified by stimulated with anti-CD3/CD28 Dynabeads for 7 days.
Qian Y., Arellano G., Ifergan I., Lin J., Snowden C., Kim T., Thomas J.J., Law C., Guan T., Balabanov R.D., Kaech S.M., Miller S.D, & Choi J. (2021). ZEB1 promotes pathogenic Th1 and Th17 cell differentiation in multiple sclerosis. Cell reports, 36(8), 109602.
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