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Dna mini kit 50

Manufactured by Qiagen
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The DNA Mini Kit 50 is a laboratory equipment product designed for the purification of DNA. It is a standard kit that provides the necessary reagents and consumables for the extraction and isolation of DNA samples.

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Lab products found in correlation

Immunoseq kit, by Adaptive Biotechnologies (1 mentions) Immunoseq, by Adaptive Biotechnologies (1 mentions)

Spelling variants of this product

Mini Kits (17 citations) QIAamp DNA Mini Kit 50 (13 citations) DNA kits (7 citations) QIAamp DNA Stool Mini Kit (50) (4 citations) AllPrep DNA/RNA Mini Kit 50 (2 citations)

2 protocols using dna mini kit 50

1

Profiling T Cell Receptor Diversity

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Total DNA was isolated from FACS-sorted T cell subsets (fetal skin and intestine) using the DNA Mini Kit 50 (51304; Qiagen). High-throughput TCR sequencing of the Vβ chain was performed using the ImmunoSEQ kit (Adaptive Biotechnologies) according to manufacturer’s instructions. CDR3 sequences of paired samples were compared to determine the frequency of common clones. TCR reads were plotted according to their Vβ family type.
Reitermaier R., Krausgruber T., Fortelny N., Ayub T., Vieyra-Garcia P.A., Kienzl P., Wolf P., Scharrer A., Fiala C., Kölz M., Hiess M., Vierhapper M., Schuster C., Spittler A., Worda C., Weninger W., Bock C., Eppel W, & Elbe-Bürger A. (2021). αβγδ T cells play a vital role in fetal human skin development and immunity. The Journal of Experimental Medicine, 218(4), e20201189.
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2

TCR Vβ Chain Sequencing Analysis

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DNA was isolated from frozen, in optimal cutting temperature (O.C.T.; Tokio) compound-embedded fetal skin samples with the DNA Mini Kit 50 (Qiagen), and used to amplify the CDR3 of the TCR Vβ chain for sequencing analysis by ImmunoSEQ (Adaptive Biotechnologies) according to the manufacturer's instructions. Circos plots were performed using R software (http://www.r-project.org/).
Reitermaier R., Ayub T., Staller J., Kienzl P., Fortelny N., Vieyra-Garcia P.A., Worda C., Fiala C., Staud C., Eppel W., Scharrer A., Krausgruber T, & Elbe-Bürger A. (2021). The molecular and phenotypic makeup of fetal human skin T lymphocytes. Development (Cambridge, England), 149(8), dev199781.
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