Ab45942

Manufactured by Abcam

Sourced in Canada, United States

Ab45942 is a laboratory product from Abcam. It is a tool used in scientific research. The core function of this product is to facilitate specific experimental procedures. No further details are available for this item while maintaining an unbiased and factual approach.

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Lab products found in correlation

3 protocols using ab45942

Mitochondrial Function Assay Protocol

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Endothelin-1 (ET1), compound C, β-actin antibody, l-carnitine hydrochloride, oligomycin, carbonylcyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP), rotenone, antimycin A, and etomoxir were from Sigma Aldrich (St Louis, MO). CB-13 and the JC-1 mitochondrial membrane potential assay kit were from Cayman Chemical (Ann Arbor, MI). Calcein-AM (Molecular Probes) and carnitine palmitoyltransferase (CPT)-1β primers were from Life Technologies (Carlsbad, CA). p-AMPKα (Thr172) and AMPKα antibodies were from Cell Signaling (2535S and 2603S, respectively; Whitby, Canada). CB1 and CB2 antibodies were from Abcam (ab23703 and ab45942, respectively; Toronto, Canada). Proliferator-activated receptor-gamma coactivator (PGC)-1α antibody was from EMD Millipore (ST1202; Temecula, CA). XF24 FluxPaks were from Agilent (Santa Clara, CA).

Lu Y., Lee D.I., Roy Chowdhury S., Lu P., Kamboj A., Anderson C.M., Fernyhough P, & Anderson H.D. (2019). Activation of Cannabinoid Receptors Attenuates Endothelin-1–Induced Mitochondrial Dysfunction in Rat Ventricular Myocytes. Journal of Cardiovascular Pharmacology, 75(1), 54-63.

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Protein Expression Analysis by Western Blot

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Protein expression was analyzed by Western blot analysis.^{57 (link)} The primary antibodies used were as follows: anti-CB2 (ab45942; Abcam), anti-fibronectin (F3648; Sigma), anti-α-SMA (A2547; Sigma, St. Louis, MO), anti-β-catenin (610154; BD Transduction Laboratories), anti-MMP-7 (3801; Cell Signaling Technologies), anti-Snail1 (ab17732; Abcam), anti-PAI-1 (sc-5297; Santa Cruz Biotechnology), anti-phospho–NF-κB p65 (Ser536) (3036; Cell Signaling Technology), and anti-NF-κB p65 (3034; Cell Signaling Technologies), anti-pSmad3 (9520; Cell Signaling Technology), p-p38 (9211; Cell Signaling Technology), p-ERK (9101; Cell Signaling Technology), p-JNK (9251; Cell Signaling Technology), TNF-α (ab9739; Abcam, Cambridge, MA), iNOS (ab15323; Abcam, Cambridge, MA), and Mannose R (ab64693; Abcam, Cambridge, MA).

Zhou L., Zhou S., Yang P., Tian Y., Feng Z., Xie X.Q, & Liu Y. (2018). Targeted Inhibition of the type 2 cannabinoid receptor is a novel approach to reduce renal fibrosis. Kidney international, 94(4), 756-772.

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Immunohistochemical Localization of CB2 Receptors

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CB₂ receptors were immunostained with the polyclonal anti-cannabinoid receptor II antibody (ab45942, Abcam, USA; dilution 1:50), using Alexa 488-conjugated anti-rabbit raised in goat (Molecular Probes–Invitrogen; dilution 1:400) as the secondary antibody. Antibodies were diluted in PBS supplemented with 1% bovine serum albumin (BSA) and 0.04% triton. All preparations were contrasted with TOPRO-3 iodide (Life Technologies, USA; dilution 1:500). Specificity was confirmed in separated experiments with additional negative controls, including tissue sections incubated with primary antibody pre-adsorbed to an excess of control peptide (ab45941, Abcam, USA; dilution 1:25).
Cochleae assigned to surface preparations were immunolabeled once bisected into two halves and before final dissection, by means of a “free floating” technique. Transversal sections were immunolabeled once mounted on the slide and conveniently dried.
As a positive control of the primary antibody used, spleen and kidneys [28 (link)] of each animal were submitted to the same protocol.

Martín-Saldaña S., Trinidad A., Ramil E., Sánchez-López A.J., Coronado M.J., Martínez-Martínez E., García J.M., García-Berrocal J.R, & Ramírez-Camacho R. (2016). Spontaneous Cannabinoid Receptor 2 (CB2) Expression in the Cochlea of Adult Albino Rat and Its Up-Regulation after Cisplatin Treatment. PLoS ONE, 11(8), e0161954.

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