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Phospho p70 s6 kinase t389 108d

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Phospho-p70 S6 Kinase T389 (108D) is a lab equipment product that is used to detect and quantify the phosphorylation of the p70 S6 kinase protein at the threonine 389 residue. It is a tool for researchers to study cell signaling pathways and protein regulation.

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Lab products found in correlation

Horseradish peroxidase coupled goat anti rabbit antibody, by Jackson ImmunoResearch (2 mentions) C myc, by Cell Signaling Technology (2 mentions) β catenin, by Cell Signaling Technology (2 mentions) P27kip1, by Cell Signaling Technology (2 mentions)

Spelling variants of this product

Phospho-S6 (110 citations) P70 S6 kinase (82 citations) Phospho-p70 S6 kinase (52 citations) Phospho-p70 S6 Kinase T389 (7 citations) Phospho-T389 S6 kinase (4 citations)

2 protocols using phospho p70 s6 kinase t389 108d

1

Immunoblotting of Cell Signaling Proteins

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Cells were lysed in RIPA buffer, immunoblotting was performed following standard procedures. The following antibodies were obtained from Cell Signaling: Cyclin D3 (DCS22), MEK1/2 (DIA5), GSK3 (D75D3), β-Catenin (polyclonal, Lot 3), c-Myc (D84C12), p27 Kip1 (polyclonal, Lot 5), phospho-p70 S6 Kinase T389 (108D2), phospho-S6 S235/236 (D57.2.2E). A horseradish peroxidase coupled goat anti-rabbit antibody (Jackson) was used as a secondary antibody. 1% ovalbumin in PBS + 0.1% Tween20 was used as blocking buffer.
Jellusova J., Cato M.H., Apgar J.R., Ramezani-Rad P., Leung C., Chen C., Richardson A.D., Conner E.M., Benschop R.J., Woodgett J.R, & Rickert R.C. (2017). GSK3 is a metabolic checkpoint regulator in B cells. Nature immunology, 18(3), 303-312.
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2

Immunoblotting of Cell Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA buffer, immunoblotting was performed following standard procedures. The following antibodies were obtained from Cell Signaling: Cyclin D3 (DCS22), MEK1/2 (DIA5), GSK3 (D75D3), β-Catenin (polyclonal, Lot 3), c-Myc (D84C12), p27 Kip1 (polyclonal, Lot 5), phospho-p70 S6 Kinase T389 (108D2), phospho-S6 S235/236 (D57.2.2E). A horseradish peroxidase coupled goat anti-rabbit antibody (Jackson) was used as a secondary antibody. 1% ovalbumin in PBS + 0.1% Tween20 was used as blocking buffer.
Jellusova J., Cato M.H., Apgar J.R., Ramezani-Rad P., Leung C., Chen C., Richardson A.D., Conner E.M., Benschop R.J., Woodgett J.R, & Rickert R.C. (2017). GSK3 is a metabolic checkpoint regulator in B cells. Nature immunology, 18(3), 303-312.
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