Secondary biotin conjugated anti rabbit or anti mouse antibodies

Manufactured by Jackson ImmunoResearch

Sourced in Panama

Secondary biotin-conjugated anti-rabbit or anti-mouse antibodies are used to detect the presence of primary antibodies raised in rabbits or mice in various research applications. These antibodies are conjugated with biotin, which can be subsequently detected using streptavidin-based detection systems.

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Lab products found in correlation

Actin, by Merck Group (1 mentions) Loricrin prb 145p, by Fortrea (1 mentions) A2066, by Merck Group (1 mentions) Ha 11, by BioLegend (1 mentions) Prb 160p, by BioLegend (1 mentions) Goat anti s100a9, by R&D Systems (1 mentions) Prb 169p, by BioLegend (1 mentions) Secondary peroxidase conjugated anti rabbit na934 and anti mouse na931 antibodies, by GE Healthcare (1 mentions) Prb 145p, by BioLegend (1 mentions) Sc 1004, by Santa Cruz Biotechnology (1 mentions) T6199, by Merck Group (1 mentions)

2 protocols using secondary biotin conjugated anti rabbit or anti mouse antibodies

Protein Analysis of SMAD Signaling

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Polyclonal antibodies against pSMAD2/3 Ser 465/467 (3108 P), SMAD2 (5339 P), and SMAD3 (9523 P) were from Cell Signaling Technology, Inc. Actin (A2066, Sigma-Aldrich, St. Louis, MO) was used as protein loading control. Secondary peroxidase-conjugated anti-rabbit antibodies were from GE HealthCare. The GILZ antibody was purchased from eBioscience (14-4033).
For immunostaining, rabbit polyclonal antibodies against K6 (PRB-169P) and loricrin (PRB-145P) were from Covance, Berkeley, CA, and Caspase-14 (sc-5628) and p-SMAD2/3 (sc-11769-R) were from Santa Cruz, CA. Secondary biotin-conjugated anti-rabbit or anti-mouse antibodies (Jackson ImmunoResearch, West Grove, PA) were used.

Carceller E., Ballegeer M., Deckers J., Riccardi C., Bruscoli S., Hochepied T., Libert C, & Pérez P. (2016). Overexpression of Glucocorticoid-induced Leucine Zipper (GILZ) increases susceptibility to Imiquimod-induced psoriasis and involves cutaneous activation of TGF-β1. Scientific Reports, 6, 38825.

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Immunoblotting Techniques for Protein Detection

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Immunoblotting was performed as described (Sevilla et al., 2013) . Mouse monoclonal antibodies used were specific for hemagglutinin (HA-11, Biolegend, San Diego, CA), and tubulin (T6199, Sigma). Rabbit polyclonal antibodies used were specific for GR (sc-1004, Santa Cruz Biotechnology, Santa Cruz, CA), actin (A-2066, Sigma), phosphorylated GR (Ser211; #4161), STAT3 (#9132), and phosphorylated STAT3 (Tyr705 D3A7; #9145) (Cell Signaling Technology, Beverley, MA). Goat anti-S100A9 was from R&D Systems (Minneapolis, MN). Secondary peroxidase-conjugated anti-rabbit (NA934) and anti-mouse (NA931) antibodies were from GE Healthcare (GE Healthcare Bio-Sciences, Pittsburgh, PA).
Rabbit polyclonal antibodies against keratin 5 (PRB-160P), keratin 6 (PRB-169P), and loricrin (PRB-145P) (Biolegend), and mouse anti-BrdU (Clone BMC 9318, Roche Diagnostics, Mannheim, Germany) were used. Secondary biotin-conjugated anti-rabbit or anti-mouse antibodies (Jackson ImmunoResearch, West Grove, PA) and secondary Alexa Fluor anti-rabbit (555, A-31572) or anti-mouse (488, A-21202) antibodies were used (Thermo Fisher, Thermo Fisher Scientific, Waltham, MA).

Boix J., Sevilla L.M., Sáez Z., Carceller E, & Pérez P. (2016). Epidermal Mineralocorticoid Receptor Plays Beneficial and Adverse Effects in Skin and Mediates Glucocorticoid Responses. The Journal of investigative dermatology, 136(12).

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