The mice were sacrificed 28 days after myocardial infarction injury. The hearts were homogenized in a RIPA buffer, the equivalent of 50 μg of protein was separated on 10% gel and then transferred to a PVDF membrane. After blocked with 5% fat-free milk, the membranes were incubated with the relevant protein antibodies overnight. The membranes were washed with TBST then treated with secondary antibodies for 2 h at room temperature. Protein extracts were separated by SDS-PAGE and detected using immunoblotting with antibodies against anti-Phospho-FGF Receptor, phospho-ERK1/2, ERK, phospho-AKT, AKT and β-actin (Cell Signaling). Horseradish peroxidase-conjugated anti-rabbit or anti-mouse secondary antibodies (Pierce) was added. Intensity of specific blots was analyzed using image J (version 1.51 and 1.52).
Anti phospho fgf receptor
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-Phospho-FGF Receptor is a primary antibody that specifically binds to the phosphorylated form of the Fibroblast Growth Factor (FGF) receptor. It can be used to detect and quantify the activation of FGF receptor signaling pathways in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Phospho akt, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Phospho erk1 2, by Cell Signaling Technology (1 mentions)
Tgf β2, by Thermo Fisher Scientific (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
Tumor necrosis factor α, by Thermo Fisher Scientific (1 mentions)
Interleukin il 1β, by Thermo Fisher Scientific (1 mentions)
Mouse monoclonal anti human cd31 antibody, by R&D Systems (1 mentions)
Anti tgf β receptor 1, by Merck Group (1 mentions)
Hrp conjugated secondary antibodies for western blot detection, by Cell Signaling Technology (1 mentions)
Imidapril, by Mitsubishi (1 mentions)
Ta 606, by Mitsubishi (1 mentions)
2 protocols using anti phospho fgf receptor
1
Myocardial Infarction Protein Analysis
2
Molecular Mechanisms of Vascular Remodeling
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The AcSDKP was a gift from Dr. Omata from Asabio Bio Technology (Osaka, Japan). Imidapril (ACE-I) and TA-606 (ARB) were provided by Mitsubishi Tanabe Pharma (Osaka Japan) through an MTA agreement. The mouse monoclonal anti-human CD31 antibody was purchased from R&D Systems (Minneapolis, MN, USA), and the rat polyclonal anti-mouse CD31 antibody was purchased from EMFRET Analytics GmbH & Co. KG (Eibelstadt, Germany). The polyclonal rabbit anti-αSMA antibody was obtained from Gene Tex (Irvine, CA, USA). The rabbit polyclonal anti-SM22α antibody and monoclonal anti-VE-cadherin antibody were obtained from Novus Biological (Littleton, CO, USA). The polyclonal anti-GAPDH and anti-TGF-β-receptor I antibodies were obtained from Sigma-Aldrich (St. Louis, MO, USA). Fluorescein-, rhodamine-, and Alexa 647-conjugated secondary antibodies were obtained from Jackson ImmunoResearch (West Grove, PA, USA). Antifibroblast growth factor (FGF) receptor, anti-phospho-FGF receptor, and the HRP-conjugated secondary antibodies for Western blot detection were purchased from Cell Signaling Technology (Danvers, MA, USA). TGF-β2, tumor necrosis factor (TNF)-α, and interleukin (IL)-1β were purchased from PeproTech (Rocky Hill, NJ, USA).
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