Cells were fixed with Cytofix Fixation Buffer (BD Biosciences, San Jose, CA), permeablized with Phosflow Perm Buffer III (BD Biosciences), then stained for 30min with optimal concentrations of PE-conjugated antibodies to p-NF-κB (Cell Signaling, Danvers, MA) or irrelevant antibodies of the same isotype and flourochrome. After staining, the cells were washed, fixed in Cytofix Fixation Buffer (BD Biosciences), and analyzed by flow cytometry. Data were analyzed with FlowJo software version 10.0.
Pe conjugated antibodies to p nf κb
Manufactured by Cell Signaling Technology
PE-conjugated antibodies to p-NF-κB are lab reagents used to detect and quantify the phosphorylated form of the transcription factor NF-κB in biological samples. These antibodies are conjugated to the fluorescent dye phycoerythrin (PE) for sensitive detection by flow cytometry or other fluorescence-based methods.
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Lab products found in correlation
2 protocols using pe conjugated antibodies to p nf κb
1
Intracellular Phospho-NF-κB Analysis
2
Intracellular Phospho-NF-κB Analysis
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