Heat shock protein (HSP) 90 antibody was from Sigma, AS160 antibody was from EMD Millipore, purified GLUT4 antiserum from Hoffmann-La Roche (Al-Hasani et al. 2002 (link)), GLUT1 antibody was from Santa Cruz, p62, S6K1, S6K1 Thr389, AS160 Thr642, PKB, PKB Ser473, PKB Thr308, and LC3 antibodies raised against LC3A/B were from Cell Signaling Technologies. Proteasomal inhibitor MG132 was from Sigma Aldrich, fluorescence-conjugated secondary antibodies Alexa Fluor-568 and BODIPY from Molecular Probe, bovine serum albumin (BSA) from Celliance (Toronto, Canada), NEC042 and NEC3770 were from Perkin Elmer, and rapamycin and chloroquine were from Enzo Life Sciences (Farmingdale, NY, USA).
Glut1 antibody
Manufactured by Santa Cruz Biotechnology
The GLUT1 antibody is a laboratory reagent used to detect the presence and distribution of the GLUT1 protein, which is a glucose transporter found in various cell types. It can be used in techniques such as Western blotting, immunohistochemistry, and flow cytometry to study glucose metabolism and transport processes.
Automatically generated - may contain errors
Lab products found in correlation
Chloroquine, by Enzo Life Sciences (1 mentions)
Rapamycin, by Enzo Life Sciences (1 mentions)
Bodipy, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568, by Thermo Fisher Scientific (1 mentions)
Nec042, by PerkinElmer (1 mentions)
Ndrg2 antibody, by Abnova (1 mentions)
Biotinylated goat anti rabbit immunoglobulin g igg, by Merck Group (1 mentions)
3 3 diaminobenzidine (dab), by Merck Group (1 mentions)
2 protocols using glut1 antibody
1
Antibodies and Reagents for Cell Signaling
2
Immunohistochemical Analysis of NDRG2 and GLUT1
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immunohistochemistry (IHC) was performed using the avidin-biotin-peroxidase complex method on all breast carcinoma samples. All sections were deparaffinised in xylenes and dehydrated through a gradient concentration of alcohol before endogenous peroxidase activity was blocked using 0.5% H2O2 in methanol for 10 minutes. After nonspecific binding was blocked, the slides were incubated with NDRG2 antibody (1:200; Abnova, Taipei, Taiwan) or GLUT1 antibody (1:200; Santa Cruz Biotechnology, Santa Cruz, CA, USA) in phosphate-buffered saline (PBS) at 4°C overnight in a humidified container. Biotinylated goat anti-rabbit immunoglobulin G (IgG) (1:400; Sigma-Aldrich, St Louis, MO, USA) was incubated with the sections for 1 hour at room temperature and detected using a streptavidin-peroxidase complex. The brown colour indicative of peroxidase activity was developed by incubation with 0.1% 3,3′-diaminobenzidine (Sigma-Aldrich) in PBS with 0.05% H2O2 for 5 minutes at room temperature. The appropriate positive and negative controls were included in each run of IHC.
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