The following primary antibodies were used for immunoblot (alphabetical order): anti-APOBEC3G (AIDS Reagent Program, Division of AIDS, NIAID, NIH from Immunodiagnostics, 10069), anti-ß-catenin (ab32572, Abcam, UK), anti-calreticulin (PA3-900, Thermo Scientific), anti-FLAG (M2, Sigma), anti-HA (16B12, Biolegend), anti-p24 (ab9071, Abcam), anti-PPP2R5A (ab89621, Abcam), anti-PPP2R5D (ab88075, Abcam), anti-PPP2R5D (EPR15617, ab188323, Abcam), anti-RRM2 (ab57653, Abcam), anti-Vif (#319, AIDS Reagent Program, Division of AIDS, NIAID, NIH: Dr. Michael Malim #6459 (Fouchier et al., 1996 (link); Simon et al., 1997 (link)).
The following primary antibodies were used for flow cytometry: anti-CD4-AF647 (clone OKT4; BioLegend, UK), anti-HA-DyLight 650 (16B12, ab117515, Abcam), anti-PPP2R5D (EPR15617, ab188323, Abcam), anti-ICAM3 (TU41, 555957, BD).
The following primary antibodies were used for immunoprecipitation anti-PPP2R5D (EPR15617, ab188323, Abcam).
The following secondary antibodies were used: goat anti-mouse-AF647 and donkey anti-rabbit-AF647 (flow cytometry, Molecular Probes, Thermo Fisher Scientific); goat anti-mouse-HRP and anti-rabbit-HRP (immunoblot, Jackson ImmunoResearch, West Grove, PA).
Greenwood E.J., Matheson N.J., Wals K., van den Boomen D.J., Antrobus R., Williamson J.C, & Lehner P.J. (2016). Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. eLife, 5, e18296.
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