All of the cell cultures were maintained in a humidified atmosphere of 5% CO2 and 95% air at 37 °C. BMSCs at passage 2 (P = 2) and ADSCs at P = 1 were purchased from Lonza. ASC52telo cells, hTERT-immortalized adipose-derived mesenchymal stem cells, were obtained from ATCC. HeLa cells were obtained from the Health Science Research Resources Bank (Osaka, Japan). BMSCs were cultured in an MSCGM BulletKit™, a mesenchymal stem cell basal medium supplemented with mesenchymal cell growth supplement, l -glutamine, and gentamycin/amphotericin-B (Lonza). ADSCs and ASC52telo cells were cultured in an ADSC-BulletKit™, an ADSC basal medium supplemented with the necessary supplements (Lonza). HeLa cells were cultured in Eagle's minimum essential medium supplemented with 10% fetal bovine serum (FBS; Sigma), 0.1 mM non-essential amino acids (Gibco), 50 U/ml penicillin, and 50 μg/ml streptomycin (Gibco). Until they were used for cell growth analysis, cells were maintained in the medium as described above and passaged upon reaching 90% confluence.
Gentamycin amphotericin b
Manufactured by Lonza
Sourced in Switzerland
Gentamycin/amphotericin-B is a broad-spectrum antibiotic and antifungal agent used in laboratory settings. It inhibits the growth of a variety of bacterial and fungal species. This product is intended for research and development purposes only.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Mscgm bulletkit, by Lonza (1 mentions)
Bmscs, by Lonza (1 mentions)
Hela cells, by Health Science Research Resources Bank (1 mentions)
Asc52telo cells, by American Type Culture Collection (1 mentions)
Adscs, by Lonza (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
L glutamine, by Lonza (1 mentions)
Adsc bulletkit, by Lonza (1 mentions)
Htert hme1, by Takara Bio (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Recombinant human vegf, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Trypsin edta, by Merck Group (1 mentions)
Singlequots, by Lonza (1 mentions)
Human epidermal growth factor (hegf), by Lonza (1 mentions)
Hfgf b, by Lonza (1 mentions)
Vascular endothelial growth factor (vegf), by Lonza (1 mentions)
3 protocols using gentamycin amphotericin b
1
Characterization of Stem Cell Cultures
2
Cell Culture Conditions Across Cell Lines
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HEK293T and HeLa cells were obtained from the ATCC and grown in DMEM (Gibco-BRL), supplemented with 5% fetal bovine serum (FBS) with penicillin (100 U/mL), and streptomycin (100 μg/mL). The human normal mammary epithelial cell line hTERT-HME1 was purchased from Clontech and grown in mammary epithelium growth media (MEGM) containing bovine pituitary extract, hydrocortisone, insulin, epithelial growth factor, and gentamycin/amphotericin-B (Lonza). U6A cells were described previously.36 (link) WT and STAT2 T403A/T403A MEFs were cultured in Dulbecco’s modified Eagle’s medium (DMEM) plus 10% fetal bovine serum (FBS). WT and STAT2 T403A/T403A BMDCs were cultured in RPMI-1640 medium plus 10% FBS. Vero cells were cultured in DMEM plus 5% FBS. All the media were supplemented with penicillin (100 IU/mL), and streptomycin (100 mg/mL). Cells were maintained in an incubator at 37 °C with 5% CO2.
3
Culturing HUVECs under Normoxia and Hypoxia
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Dimethylsulfoxide (DMSO), fetal bovine serum (FBS), antibiotics (penicillin and streptomycin), trypsin-EDTA, and human recombinant VEGF were obtained from Sigma Aldrich (St. Louis, MO, USA). HUVECs were purchased from Lonza (Basel, Switzerland), cultured in EGM complete medium supplemented with SingleQuots™ (containing hydrocortisone, hEGF, FBS, VEGF, hFGF-B, R3-IGF-1, ascorbic acid, heparin and gentamycin/amphotericin-B, Lonza) and incubated at 37°C and 5% CO2 in normoxia (21% O2) or hypoxia (2.5% O2). The hypoxia was guarantee by the use of the hypoxic station InVivo2 200 (Baker Ruskinn, Sanford, MA, USA). To maintain the exponential growth, cells were divided when they reached 80% of confluence in a 25 cm 2 dish. HUVECs at passage between 3 and 8 were used for the experiments.
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