A human ovarian tumor array was purchased from US Biomax (OV8011). The paraffin-embedded slides were baked, deparaffinized in Americlear (Adwin Scientific, 72060044), and rehydrated in decreasing concentrations of ethanol. Antigen retrieval was performed by incubation in heated Dako target retrieval solution. Slides were then permeabilized by adding Triton-X100 (0.15%) to wash solution, blocked in 50% horse serum, and incubated overnight with anti–p-ERK primary antibody (Cell Signaling, 4370) or isotype control per manufacturer's recommendation. Slides were washed and incubated with an anti-rabbit secondary antibody conjugated to Dylight 488 (Invitrogen, 35,552). Tissue sections on the slide were immersed in Vectashield with DAPI and coverslipped. Slides were imaged at 60× on an Olympus BX43F fluorescence microscope utilizing cellSens software.
Anti p erk primary antibody
Manufactured by Cell Signaling Technology
The Anti–p-ERK primary antibody is a laboratory reagent used to detect and quantify the phosphorylated form of extracellular signal-regulated kinase (ERK), a key signaling protein involved in cellular processes. This antibody specifically recognizes the phosphorylated epitopes of ERK, enabling researchers to study ERK activation in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Dylight 488, by Thermo Fisher Scientific (1 mentions)
Bx43f fluorescence microscope, by Olympus (1 mentions)
Protease and phosphatase inhibitor cocktail, by MedChemExpress (1 mentions)
Anti β actin primary antibody, by Abcam (1 mentions)
Anti erk primary antibody, by Cell Signaling Technology (1 mentions)
Clarity western ecl substrate, by Bio-Rad (1 mentions)
Bca assay kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti p erk primary antibody
1
Immunohistochemical Analysis of p-ERK in Ovarian Tumor
2
Comprehensive Western Blot Analysis Protocol
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Western blot analysis was performed as previously described [4 (link)]. Antibodies used in this study were shown as below: Anti-β-actin primary antibody was purchased from Abcam and served as an internal control((cat. No.ab115777). In brief, protein extracts were prepared by lysing cells or tissues in RIPA buffer containing protease and phosphatase inhibitor cocktails (MedChem Express). Protein concentrations were quantified with a BCA assay kit (Thermo Scientific). Equal amounts of protein were denatured by boiling, separated by SDS-polyacrylamide gel electrophoresis, and transferred to polyvinylidene fluoride (PVDF) membranes. The membranes were blocked with 5% skimmed milk and then incubated with primary antibodies followed by HRP-conjugated secondary antibodies. Chemiluminescent signals were detected using ClarityTM Western ECL Substrate (Bio-Rad).Anti-ARNT primary antibody was purchased from Abcam(cat. No. ab270520). Anti-p38α primary antibody was purchased from Abcam (cat. No. ab170099). Anti-p-ERK primary antibody was purchased from Cell signaling technology(cat. No. #8544). Anti-ERK primary antibody was purchased from Cell signaling technology (cat. No. #4695).
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