Sst gel clot activator tube

Blood was taken from the abdominal vein and collected in an SST gel & clot activator tube (Becton and Dickinson, Franklin Lakes, NJ, USA). The serum was separated by centrifugation at 1,500g for 10 min at room temperature. Serum biochemical parameters such as serum glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), albumin concentration (ALB), serum total cholesterol (T-CHO), low-density lipoprotein-cholesterol (LDL-C), triglyceride (TG), and glucose (GLU) were analyzed using their respective commercially available kits (Diagnostic Product Corporation, Los Angeles, CA, USA).

To measure the blood chemistry parameters, blood samples were collected from the abdominal vein in a SST® gel & clot activator tube (Becton and Dickinson, Franklin Lakes, NJ, USA). Serum was separated by centrifugation at 1500 × g for 10 min at room temperature. An automated chemistry analyzer (Hitachi-747, Hitachi Medical Co., Tokyo, Japan) was used to measure serum biochemical parameters association with lipid metabolism and liver functions including the aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), glutamyl transpeptidase (γ-GTP), albumin (Alb), total cholesterol (T-Cho), triglyceride (TG), high-density lipoprotein-cholesterol (HDL-C), and low-density lipoprotein-cholesterol (LDL-C). A part of blood was collected in a test tube containing ethylenediaminetetraacetic acid (EDTA) (Vacutainer K3E, BD Biosciences, Plymouth, UK) as an anticoagulant to measure the number of blood cell and analyzed using an automated blood cell analyzer (Sysmex NE-8000, TOA Medical Electronics, Kobe, Japan).