Atomic force microscope (Asylum Research, Santa Barbara, CA, USA) was used to analyze 15 µm sections of the tendon tissue from different groups. Micromorphological imaging of the tendon tissue was performed using a silicon probe (PPP‐NCLR‐20, Nanosensors, Neuchatel, Switzerland) with a force constant of 42 N/m and a resonance frequency of 161 kHz after the hydrated sections were naturally dried. All measurements were repeated for three positions of each tissue sample and the values were averaged.
Ppp nclr 20
Manufactured by Nanosensors
Sourced in Switzerland
The PPP-NCLR-20 is a laboratory equipment product. It is a type of nanoscale cantilever sensor.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using ppp nclr 20
1
Atomic Force Microscopy Analysis of Tendon
2
AFM Characterization of Fresh Tissue
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An AFM (Keysight 5500, Keysight Technologies, Santa Rosa, CA, USA) was used to analyze 20 μm thick fresh frozen tissue sections without decalcification. For the micromorphological imaging, after the sections were washed with water to remove the embedding medium (O.C.T compound) and dried naturally. A silicon probe (PPP-NCLR-20, Nanosensors™, Neuchatel, Switzerland) with a force constant of 42 N/m and a resonance frequency of 161 kHz was used for imaging. For measurement of mechanical properties, the AFM-based nanoindentation was used. The washed specimens were immersed in physiological saline at room temperature, and a silicon oxide tip with 13 kHz resonance frequency and 0.2 N/m force constant (SD-Sphere-CONT-M-10, Nanosensors) was used in the contact mode. The elastic modulus of each position was measured at an indentation depth of 300 nm. All measurements were repeated for six positions in each tissue specimen, and the values were averaged.
3
Atomic Force Microscopy of Paraffin Sections
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Paraffin sections were analyzed using an atomic force microscope (Keysight 5500; Keysight Technologies, Santa Rosa, CA, USA) operating in tapping mode. Images were captured using a silicon probe (PPP-NCLR-20; Nanosensors, Neuchatel, Switzerland) with a 42-N/m force constant and a 161-kHz resonance frequency. To assess mechanical properties, a probe (SD-Sphere-CONT-m-10; Nanosensors) with a 0.2-N/m force constant and a 13-kHz resonance frequency was used, achieving a 300-nm indentation depth at each section position. Six different locations within each tissue sample were measured and then averaged for analysis.
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