Agilent 6410 triple quadrupole lc ms system

Buds of A. annua were dried at 45 C and pestled into fine powder, extracted with 1.5 ml of ethanol/0.1 g of sample. The suspension was supersonic for 30 min and then centrifuged at 5000 rpm for 10 min to remove the suspended particles (Lu et al., 2013) . The final supernatant was filtered through a 0.25-mm pore size filter. Contents of artemisinin, AA, and DHAA were determined by LC-MS/MS. LC-MS/MS analysis was run on an Agilent 1200 infinity LC column coupled with an Agilent 6410 Triple Quadrupole LC/MS System (Agilent, USA). The LC operating conditions were as follows: LC column, ZORBAX SB-C18, 2.1 3 100 mm i.d., 3.5 mm silica; mobile phase, acetonitrile/0.1% formic acid (70:30); total flow rate of mobile phase, 0.3 ml/min; total run time including equilibration, 4.2 min. The injection volume was 5 ml. The MS/MS was operated with an electrospray ionization source in positive ion mode with multiple reaction monitoring. The nebulizer gas pressure was set at 40 psi with a source temperature of 350 C and gas flow at 10 l/min. The capillary voltage was 4000 V (positive mode). High-purity nitrogen gas was used as collision cell gas. The raw chromatograph and mass spectrogram data were processed with the MassHunter Workstation software (Agilent, USA).