In chow-fed mice, plasma albumin, bilirubin, blood urea nitrogen, creatinine, ALT, and AST levels were measured using the AU480 Clinical Chemistry Analyzer (Beckman Coulter, Providence, RI). In high-fat–fed mice, plasma ALT and AST activity was measured using the ALT Activity Assay Kit (700260; Cayman Chemical, Ann Arbor, MI) and the AST Activity Assay Kit (MAK055; Sigma-Aldrich, St. Louis, MO), respectively. Fasting blood glucose and plasma insulin were assessed using the Accu-Chek glucometer (Roche Diagnostics, Basel, Switzerland) and the Ultrasensitive Mouse Insulin Enzyme-Linked Immunosorbent Assay Kit (90080; Crystal Chem, Downers Grove, IL), respectively. TAG and collagen content were assessed in liver homogenate using the Triglyceride Quantification Colorimetric Kit (K622-100; BioVision, Mountain View, CA) and the Hydroxyproline Colorimetric Assay Kit (MAK008; Sigma-Aldrich), respectively. To characterize hepatic oxidative stress, 4-HNE and TBARS levels in liver homogenate were measured with the OxiSelect HNE Adduct Competitive Enzyme-Linked Immunosorbent Assay Kit (STA-838; Cell Biolabs, Inc, San Diego, CA) and the Lipid Peroxidation Assay Kit (MAK085; Sigma-Aldrich), respectively.
Alt activity assay kit
Manufactured by Cayman Chemical
The ALT Activity Assay Kit is a laboratory tool used to measure the activity of the enzyme alanine aminotransferase (ALT) in biological samples. ALT is an important enzyme involved in amino acid metabolism and its activity can provide insights into various physiological and pathological conditions.
Automatically generated - may contain errors
Lab products found in correlation
Ast activity assay kit, by Merck Group (2 mentions)
Lipid peroxidation assay kit, by Merck Group (1 mentions)
Hydroxyproline colorimetric assay kit, by Merck Group (1 mentions)
Ultrasensitive mouse insulin enzyme linked immunosorbent assay kit, by Crystal Chem (1 mentions)
Au480 clinical chemistry analyzer, by Beckman Coulter (1 mentions)
Triglyceride quantification colorimetric kit, by Abcam (1 mentions)
Accu chek, by Roche (1 mentions)
Triglyceride assay kit, by Cayman Chemical (1 mentions)
Glucose assay kit, by Cayman Chemical (1 mentions)
Total cholesterol assay kit, by Cell Biolabs (1 mentions)
Hydroxyproline assay kit, by Merck Group (1 mentions)
Total cholesterol and cholesteryl ester colorimetric assay kit 2, by Abcam (1 mentions)
Quantikine elisa kit, by R&D Systems (1 mentions)
3 protocols using alt activity assay kit
1
Metabolic and Liver Function Assessments
2
Plasma Lipid and Enzyme Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were collected from non-fasted mice by a submandibular bleed in EDTA capillary tubes and spun for 10 minutes at 1200 × g for 10 minutes. Plasma fraction was collected from the tube, placed into a separate microcentrifuge tube, and stored at −80 °C. Plasma cholesterol, triglycerides, and glucose concentrations were assayed and calculated with a Total Cholesterol Assay Kit (#STA-384, Cell Biolabs Inc, San Diego, CA), Triglyceride Assay Kit (#10010303, Cayman Chemical, Ann Arbor, MI), and Glucose Assay Kit (#10009582, Cayman Chemical, Ann Arbor, MI). Plasma ALT activity was quantified using an ALT Activity Assay Kit (#700620, Cayman Chemical, Ann Arbor, MI). We followed the protocols provided by the manufacturer.
3
Measuring Plasma PCSK9, Cholesterol, and Liver Parameters
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma levels of human and mouse PCSK9 were measured using the human and mouse PCSK9 Quantikine ELISA Kits, respectively (R&D Systems, Minneapolis, MN). Plasma cholesterol was assessed using the Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II (BioVision, Mountain View, CA). Plasma lipoproteins were separated in pooled samples from each group by size-exclusion chromatography, and cholesterol was quantified with a system of detection online as described. 29 (link) The plasma alanine and aspartate aminotransferase (ALT/AST) levels were determined using the ALT Activity Assay Kit (Cayman Chemical, Ann Arbor, MI) and AST Activity Assay Kit (Sigma-Aldrich), respectively. Liver lipids were extracted using the BUME (butanol/methanol) method 30 (link) and quantified using straight-phase high-performance liquid chromatography as described previously. 31 To measure hepatic collagen content, liver samples were homogenized in distilled water, hydrolyzed in 12 N HCl for 3 hours at 120°C, and analyzed using a Hydroxyproline Assay Kit (Sigma-Aldrich).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!