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Mmessage mmachine sp6 t7 transcription kits

Manufactured by Thermo Fisher Scientific
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The MMessage mMachine SP6 & T7 Transcription Kits are laboratory tools for in vitro transcription. They provide the necessary components to synthesize RNA from DNA templates using the SP6 or T7 RNA polymerase.

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MMESSAGE mMACHINE SP6/T7 kit MMESSAGE mMACHINE SP6 Transcription Kit MMESSAGE mMACHINE T7 Transcription Kit MMessage mMachine SP6 kit MMESSAGE mMACHINE T7 kit MMessage mMachine transcription kit MMachine SP6 Transcription Kit MMessage SP6 transcription kit MMACHINE T7 transcription kit SP6 mMessage mMachine

2 protocols using mmessage mmachine sp6 t7 transcription kits

1

Clonal Vangl2 Expression in Zebrafish

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Capped sense mRNA was generated from linearized plasmid using the mMessage mMachine SP6 & T7 Transcription Kits (Invitrogen). mRNA was microinjected at the 16-cell stage at 200 ng/µl, to generate clonal expression, then imaged live intact embryos from 50% epiboly. GFP-Vangl2 DNA, and mRNA was microinjected at 2–4 cell for clonal expression for in situ experiments.
Brunt L., Greicius G., Rogers S., Evans B.D., Virshup D.M., Wedgwood K.C, & Scholpp S. (2021). Vangl2 promotes the formation of long cytonemes to enable distant Wnt/β-catenin signaling. Nature Communications, 12, 2058.
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2

Clonal Expression of Capped Sense mRNA

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Capped sense mRNA was generated from linearized plasmid using the mMessage mMachine SP6 & T7 Transcription Kits (Invitrogen). mRNA was microinjected at the 16-cell stage at 200ng/µl, to generate clonal expression when imaged from 50% epiboly. GFP-Vangl2 DNA and mRNA was microinjected at 2-4 cell for clonal expression for in situ experiments.
Brunt L., Greicius G., Evans B.D., Virshup D.M., Wedgwood K.C.A., & Scholpp S. (2020). Vangl2 regulates the dynamics of Wnt cytonemes in vertebrates.
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