Ft 710

Various NMR spectra were obtained using an INOVA 600 MHz spectrometer (Agilent Technologies, Santa Clara, CA, USA). FAB-MS spectra were recorded on a mass spectrometer (JMS-700 Mstation; JEOL, Tokyo, Japan). UV spectra were recorded on a spectrophotometer (Beckman DU640 spectrophotometer; Beckman Coulter, Inc., Fullerton, CA, USA). IR spectra were recorded on a Fourier transform infrared spectrometer (FT-710; Horiba Ltd, Kyoto, Japan).

General Experimental Procedures EI-MS and FAB-MS were measured on a JMS-MS 700 mass spectrometer (JEOL, Tokyo, Japan). 1 (link) H-and 13 C-NMR spectra were recorded on a JNM-AL-400 NMR spectrometer (JEOL) at 400 MHz for 1 H and 100 MHz for 13 (link) C in CDCl 3 (δ H 7.24, δ C 77.0). Specific rotations were obtained with the digital polarimeter P-2300 (JASCO, Tokyo, Japan). UV spectra were measured on the UV-visible (Vis) spectrophotometer U-3310 (Hitachi High Technologies Co., Ltd., Tokyo, Japan). ECD spectra were measured with a JASCO J-720 spectropolarimeter. IR spectra were recorded on the Fourier transform infrared spectrometer FT-710 (Horiba Ltd., Kyoto, Japan). Preparative HPLC was performed using an L-6200 HPLC system (Hitachi High Technologies Co., Ltd.).
Materials PTP1B was purchased from Enzo Life Sciences (Farmingdale, NY, U.S.A.). p-Nitrophenyl phosphate (pNPP) was purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.). Oleanolic acid was purchased from Tokyo Chemical Industry (Tokyo, Japan). Plastic plates (96-well) were purchased from Corning Inc. (Corning, NY, U.S.A.). All other chemicals including organic solvents were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).