All chemicals were obtained from Alfa Aesar and Sigma-Aldrich. SMP-II Digital Melting Point Appliances are used to determined melting points and are uncorrected. 13C-NMR and 1H-NMR spectra were recorded in DMSO-d6 and were performed on two NMR instruments. The first was a Bruker 500J MHz-Avance III High-Performance Digital FT-NMR spectrometer at the Faculty of Science, Department of Chemistry, the University of Jordan, Jordan. The second was a Bruker 300 MHz-Avance III High-Performance Digital FT-NMR spectrometer at the NMR facility at the Doping and Narcotics Analysis Laboratory of the Faculty of Pharmacy, Anadolu University, Turkey. Tetramethylsilane was used as the internal standard. All chemical shifts were recorded as d (ppm). High-resolution mass spectra data (HRMS) were collected using a Waters LCT Premier XE Mass Spectrometer (high sensitivity orthogonal acceleration time-of-flight instrument) using the ESI (+) method (the instrument was coupled to an AQUITY Ultra Performance Liquid Chromatography system (Waters Corporation, Milford, MA, USA)) at Pharmacy Faculty, Gazi University, Ankara, Turkey.
Aquity ultra performance liquid chromatography system
Manufactured by Waters Corporation
Sourced in United States
The ACQUITY Ultra Performance Liquid Chromatography (UPLC) system is a high-performance liquid chromatography instrument designed for analytical and preparative separations. It utilizes advanced technology to provide efficient and rapid separations of complex mixtures. The system features a high-pressure solvent delivery system, an autosampler, and a variety of detection options to suit various application requirements.
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Lab products found in correlation
Lct premier xe mass spectrometer, by Waters Corporation (3 mentions)
500 j mhz avance 3 high performance digital ft nmr spectrometer, by Bruker (1 mentions)
Ft nmr mercury 400 mhz spectrometer, by Agilent Technologies (1 mentions)
Chns 932 analyzer, by Leco (1 mentions)
Spectrum 400, by PerkinElmer (1 mentions)
Silica gel 60 f254, by Merck Group (1 mentions)
Redisep column, by Teledyne (1 mentions)
Combiflash rf, by Teledyne (1 mentions)
4 protocols using aquity ultra performance liquid chromatography system
1
Spectroscopic Characterization of Organic Compounds
2
Lipidomic Analysis of Mouse Tissues
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Total lipids from mouse tissues were extracted according to the Folch method (43 (link)). Lipid extracts were analyzed by ultra-performance liquid chromatography-quadrupole time of flight-mass spectrometry as previously described (44 ). In brief, samples were separated using an Aquity Ultra-Performance Liquid Chromatography System (Waters, Milford, MA, USA) equipped with a BEH-C18-column, 2.1 × 150 mm, 1.7 μm (Waters). The gradient started from 55% solvent A (methanol/water 1/1, v/v) and 45% solvent B (isopropanol), both containing phosphoric acid (8 μM), ammonium acetate (10 mM), and formic acid (0.1 vol%) and reached 100% solvent B within 32 min at a flow rate of 150 μl/min. A Synapt G1 quadrupole time of flight high-definition mass spectrometer (Waters) equipped with an electrospray ionization source was used for analysis in positive and negative ionization mode. Data analysis was performed using the Lipid Data Analyzer software (http://genome.tugraz.at/lda2/lda_description.shtml ) (45 (link)).
3
Characterization of Organic Compounds
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All chemicals and solvents used were of reagent grade (Merck, Darmstadt, Germany; Sigma-Aldrich, St. Louis, MO, USA) and were used without further purification. All cell culture reagents were purchased from Gibco Life Technologies (Carlsbad, CA, USA).
Melting points were determined in an Electrothermal 9200 melting point apparatus and are uncorrected. Infrared (IR) spectra were obtained using a Perkin Elmer Spectrum 400 FTIR/FTNIR spectromer equipment with a Universal ATR Sampling Accessory in the range of 4000-400 cm -1 . For the region of 400-200 cm -1 , the samples were analyzed with a FTIR IFS 66/S spectrometer. Elemental analyses were performed with a Leco 932 CHNS analyzer, and proton magnetic resonance ( 1 H-NMR) spectra were recorded in dimethylsulfoxide (DMSO)d 6 (Merck) on Varian Mercury 400 MHz FT NMR spectrometer. High resolution mass spectra data (HRMS) were collected in-house using a Waters LCT Premier XE mass spectrometer (high sensitivity orthogonal acceleration time-of-flight instrument) operating in ESI (+) (positive ion mode), coupled to an AQUITY ultra performance liquid chromatography system (Waters Corporation, Milford, MA, USA). Thin-layer chromatography (TLC) was performed on precoated aluminum plates (Silicagel 60 F 254 , Merck). Plates were visualized by ultraviolet light, Dragendorff reagent, or iodine vapor.
Melting points were determined in an Electrothermal 9200 melting point apparatus and are uncorrected. Infrared (IR) spectra were obtained using a Perkin Elmer Spectrum 400 FTIR/FTNIR spectromer equipment with a Universal ATR Sampling Accessory in the range of 4000-400 cm -1 . For the region of 400-200 cm -1 , the samples were analyzed with a FTIR IFS 66/S spectrometer. Elemental analyses were performed with a Leco 932 CHNS analyzer, and proton magnetic resonance ( 1 H-NMR) spectra were recorded in dimethylsulfoxide (DMSO)d 6 (Merck) on Varian Mercury 400 MHz FT NMR spectrometer. High resolution mass spectra data (HRMS) were collected in-house using a Waters LCT Premier XE mass spectrometer (high sensitivity orthogonal acceleration time-of-flight instrument) operating in ESI (+) (positive ion mode), coupled to an AQUITY ultra performance liquid chromatography system (Waters Corporation, Milford, MA, USA). Thin-layer chromatography (TLC) was performed on precoated aluminum plates (Silicagel 60 F 254 , Merck). Plates were visualized by ultraviolet light, Dragendorff reagent, or iodine vapor.
4
Analytical Characterization of Organic Compounds
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Melting points were determined with an SMP-II Digital Melting Point Apparatus and are uncorrected (Schorpp Geaetetechnik, Germany). IR spectra were obtained using a Perkin Elmer Spectrum 400 FTIR/FTNIR spectrometer equipped with a Universal ATR Sampling Accessory. 1 High resolution mass spectra data (HRMS) were collected using a Waters LCT Premier XE Mass Spectrometer (high sensitivity orthogonal acceleration time-of-flight instrument) using ESI (þ) method. The instrument was coupled to an AQUITY Ultra Performance Liquid Chromatography system (Waters Corporation, Milford, MA, USA). Elemental analyses were performed with a LECO- 932 (C, H, N, S-Elemental Analyzer) at the Faculty of Pharmacy, Ankara University. Flash chromatography was performed with a Combiflash ® Rf automated flash chromatography system with RediSep columns (Teledyne-Isco, Lincoln, NE, USA) using DCM-MeOH, hexane-EtOAc or MeOH-EtOAc solvent gradients.
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