Hepatocyte growth factor (hgf)

Manufactured by Solarbio

Sourced in China

HGF is a laboratory reagent that functions as a growth factor. It is used in cell culture experiments to promote the proliferation and migration of various cell types.

Automatically generated - may contain errors

Lab products found in correlation

Spectramax m2 microplate reader, by Molecular Devices (1 mentions) Minute plasma membrane derived lipid raft isolation kit, by Invent Biotechnologies (1 mentions)

2 protocols using hepatocyte growth factor (hgf)

Quantifying CAF-Secreted HGF and FGF7

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The levels of HGF and FGF7 secreted by CAFs were measured via ELISA. After 48 h of culture, culture supernatants of CAFs were collected and then immediately frozen and preserved at −80 °C. Supernatants were thawed on ice, and the presence of HGF and FGF7 was detected using the HGF (Solarbio, China) and FGF7 Human ELISA Kit (PYRAM, China). HGF and FGF7 were further normalized to IGFBP-6, a stably expressed cytokine with levels directly proportional to the raw count of CAF cells. The ELISA data were collected on a SpectraMax M2 Microplate Readers (Molecular Devices).

Zhang Y., Hu Q., Pei Y., Luo H., Wang Z., Xu X., Zhang Q., Dai J., Wang Q., Fan Z., Fang Y., Ye M., Li B., Chen M., Xue Q., Zheng Q., Zhang S., Huang M., Zhang T., Gu J, & Xiong Z. (2024). A patient-specific lung cancer assembloid model with heterogeneous tumor microenvironments. Nature Communications, 15, 3382.

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GD2-Targeted Lipid Raft Isolation

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First, the transfected cells were treated with the anti-GD2 antibody ch14.18/CHO (dinutuximab beta, 1000 ng/ml, Rentschler Biopharma SE, Germany), disialoganglioside GD2 (NH4 + salt) (1000 ng/ml, Matreya LLC, USA), and recombinant mouse hepatocyte growth factor (HGF, 40 ng/ml, Solarbio, China). Then, lipid rafts were extracted using the Minute Plasma Membrane-Derived Lipid Raft Isolation Kit (Invent Biotechnologies, USA) according to the manufacturer’s instructions.

Sha Y.L., Liu Y., Yang J.X., Wang Y.Y., Gong B.C., Jin Y., Qu T.Y., Xia F.T., Han L, & Zhao Q. (2022). B3GALT4 remodels the tumor microenvironment through GD2-mediated lipid raft formation and the c-met/AKT/mTOR/IRF-1 axis in neuroblastoma. Journal of Experimental & Clinical Cancer Research : CR, 41, 314.

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