The MRSA was ground with liquid nitrogen and its metabolites analysis was carried out to analyze using a Vanquish UHPLC system coupled with an Orbitrap Q Exactive series mass spectrometer (Thermo Fisher, USA). The analytical conditions and quanti ed as a previously described study. Metabolites were relatively quanti ed by calculating the area of each individual peak with signi cant differences in content were set with thresholds of variable importance in projection (VIP) ≥1 and fold change ≥1.5 or ≤0.67. Six replicates were performed in metabolomics analysis, which was carried out by Novogene.
Orbitrap q exactive series
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Orbitrap Q Exactive series is a high-resolution, accurate-mass (HRAM) mass spectrometry platform. It combines a quadrupole mass filter with an Orbitrap mass analyzer, enabling high-performance ion detection and analysis.
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Lab products found in correlation
2 protocols using orbitrap q exactive series
1
MRSA Metabolomic Profiling via UHPLC-MS
2
Colonic Metabolite Profiling by UHPLC-MS
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The procedure of microbial metabolite measurement as our previously describe [19] using Vanquish UHPLC system (Thermo Fisher) coupled with an Orbitrap Q Exactive series mass spectrometer (Thermo Fisher). Brie y, the homogenate of grounded colonic digesta was centrifuged. The obtained supernatant was diluted with LC-MS grade water and centrifuged for 10 min, and nally injected into the LC-MS/MS system analysis. Raw UHPLC-MS/MS data was analyzed by Compound Finder 3.1 (CD3.1, Thermo Fisher Scienti c Waltham, MA, USA) to peak alignment, peak pick-up, and quanti cation of each metabolite.
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