RPMI-1640, FBS, and Pencillin/Streptomycin/Amphotercin B (PSA) were purchased from CellGro; IMDM medium was purchased from Thermo Scientific; OptiMEM was purchased from Invitrogen; Puromycin (Sigma-Aldrich, Inc.); Quantikine human osteopontin ELISA kit (R & D Systems, Inc.); Rabbit anti-osteopontin (Rockland Immunochemicals, Inc. Limerick, PA); molecular biology-grade reagents (Sigma); Trizol and PureLink RNA extraction kit (Life Technologies); Nano 6000 RNA analysis reagents (Agilent); PCR primers and TaqMan FAST Universal PCR Master Mix (Life Technologies); BD Biocoat Matrigel Invasion Chamber (BD Biosciences); Recombinant OPN (R & D Systems, Inc.).
Molecular biology grade reagents
Manufactured by Merck Group
Sourced in United States
Molecular biology-grade reagents are specialized laboratory chemicals designed for use in molecular biology research and applications. These reagents are of high purity and quality, ensuring consistent and reliable performance in techniques such as DNA and RNA analysis, gene expression studies, and protein research. They are formulated to meet the stringent requirements of molecular biology workflows.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Corning (1 mentions)
Recombinant opn, by R&D Systems (1 mentions)
Biocoat matrigel invasion chamber, by BD (1 mentions)
Imdm medium, by Thermo Fisher Scientific (1 mentions)
Human osteopontin quantikine elisa kit, by R&D Systems (1 mentions)
Taqman fast universal pcr master mix, by Thermo Fisher Scientific (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Corning (1 mentions)
Puromycin, by Merck Group (1 mentions)
Brdu incorporation assay kit, by Cell Signaling Technology (1 mentions)
Cell counting kit 8 cck 8 assay kit, by Dojindo Laboratories (1 mentions)
Tgf β1, by R&D Systems (1 mentions)
Fibroblast growth factor 2 (fgf2), by R&D Systems (1 mentions)
2 protocols using molecular biology grade reagents
1
Osteopontin Pathway Analysis Protocol
2
Quantifying Cell Responses to Growth Factors
Check if the same lab product or an alternative is used in the 5 most similar protocols
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We used the recombinant mouse OPN, TGFβ1, and FGF2 supplied by R&D Systems (Minneapolis, MN, USA). Recombinant human bone morphogenetic protein 2 (BMP2) was purchased from CowellMedi (Busan, South Korea). Quantikine Mouse/Rat osteopontin, Mouse/Rat/Porcine/Canine TGFβ1, and Mouse/Rat FGF basic ELISA kits were obtained from R&D Systems. Cell Counting Kit-8 (CCK-8) assay kit was supplied by Dojindo Laboratories (Kumamoto, Japan), and BrdU incorporation assay kit was ordered through Cell Signaling Technology (Danvers, MA, USA). The molecular biology-grade reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless stated otherwise.
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